Cloning of the cyo locus encoding the cytochrome o terminal oxidase complex of Escherichia coli

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · J. Bacteriol.; (United States)

OSTI ID:6031517

Au, D C.T.; Gennis, R B

The structural genes encoding the cytochrome o terminal oxidase complex (cyo) of Escherichia coli have been subcloned into the multicopy plasmid pBR322 after the Mu-mediated transposition of the gene locus from the bacterial chromosome onto the conjugative R plasmid RP4. Introduction of cyo plasmids into strains (cyo cyd) lacking both terminal oxidases restored the ability of the strains to grow aerobically on nonfermentable substrates. Strains carrying the cyo plasmids produced 5 to 10 times more cytochrome o oxidase than did control strains. The gene products encoded by the cyo plasmids could be immunoprecipitated with monospecific antibodies raised against cytochrome o. The cloned genes will be valuable for studying the structure, function, and regulation of the cytochrome o terminal oxidase complex.

• Research Organization: Univ. of Illinois, Urbana
• DOE Contract Number: AC02-80ER10682
• OSTI ID: 6031517
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 169:7; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CELL CONSTITUENTS
CLONING
CYTOCHROME OXIDASE
DNA-CLONING
ENZYMES
ESCHERICHIA COLI
GENES
GENETIC MAPPING
HAEM DEHYDROGENASES
MAPPING
MICROORGANISMS
OXIDOREDUCTASES
PLASMIDS