Isolation and characterization of an Escherichia coli mutant lacking the cytochrome o terminal oxidase

Journal Article · Tue Jan 01 04:00:00 EST 1985 · J. Bacteriol.; (United States)

OSTI ID:5666306

Au, D C.T.; Lorence, R M; Gennis, R B

A respiration-deficient mutant of Escherichia coli has been isolated which is unable to grow aerobically on nonfermentable substrates such as succinate and lactate. Spectroscopic and immunological studies showed that this mutant lacks the cytochrome o terminal oxidase of the high aeration branch of the aerobic electron transport chain. This strain carries a mutation in a gene designated cyo which is cotransducible with the acrA locus. Mutations in cyo were obtained by mutagenizing a strain that was cyd and, thus, was lacking the cytochrome d terminal oxidase. Strain RG99, which carries both the cyd/sup -/ and cyo/sup -/ alleles, grows normally under anaerobic conditions in the presence of nitrate. Introduction of the cyd/sup +/ allele into the strain restores the respiration function of the strain, indicating that the cytochrome o branch of the respiratory chain is dispensable under normal laboratory growth conditions.

• Research Organization: Univ. of Illinois, Urbana
• DOE Contract Number: AC02-80ER10682
• OSTI ID: 5666306
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 161:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANAEROBIC CONDITIONS
BACTERIA
BIOSYNTHESIS
CHEMICAL COMPOSITION
CYTOCHROME OXIDASE
CYTOCHROMES
ENZYMES
ESCHERICHIA COLI
GENES
GENETIC MAPPING
GROWTH
HAEM DEHYDROGENASES
MAPPING
MICROORGANISMS
MUTANTS
MUTATIONS
OXIDOREDUCTASES
PIGMENTS
RESPIRATION
SYNTHESIS