Proflavin and microwave radiation: Absence of a mutagenic interaction

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Bioelectromagnetics (New York); (USA)

DOI:https://doi.org/10.1002/bem.2250110206· OSTI ID:6165665

Meltz, M L; Eagan, P; Erwin, D N ^[1]

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1. Univ. of Texas Health Science Center, San Antonio (USA)

The potential ability of radiofrequency electromagnetic radiation (RFR) in the microwave range to induce mutagenesis, chromosomal aberrations, and sister chromatid exchanges in mammalian cells is being explored in our laboratories. In addition, we have also been examining the ability of simultaneous exposure to RFR and chemical mutagens to alter the genotoxic damage induced by chemical mutagens acting alone. We have performed experiments to determine whether there is an interaction between 2.45-GHz, pulsed-wave, RFR and proflavin, a DNA-intercalating drug. The endpoint studied was forward mutation at the thymidine kinase locus in L5178Y mouse leukemic cells. Any effect on the size distribution of the resulting colonies of mutated cells was also examined. The exposures were performed at net forward powers of 500 or 600 W, resulting in a specific absorption rate (SAR) of approximately 40 W/kg. The culture-medium temperature reached a 3 degrees C maximal increase during the 4-h exposure; appropriate 37 degrees C and convection-heating temperature controls (TC) were performed. In no case was there any indication of a statistically significant increase in the induced mutant frequency due to the simultaneous exposure to RFR and proflavin, as compared with the proflavin exposures alone. There was also no indication of any change in the colony-size distribution of the resulting mutant colonies, neither, and there was no evidence in these experiments of any mutagenic action by the RFR exposure alone.

• OSTI ID: 6165665
• Journal Information: Bioelectromagnetics (New York); (USA), Journal Name: Bioelectromagnetics (New York); (USA) Vol. 11:2; ISSN BLCTD; ISSN 0197-8462
• Country of Publication: United States
• Language: English

Related Subjects

560400* -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACRIDINES
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
AZINES
BIOLOGICAL EFFECTS
CHROMOSOMAL ABERRATIONS
DISEASES
ELECTROMAGNETIC RADIATION
ENVIRONMENTAL EXPOSURE
FLAVINES
HEMIC DISEASES
HETEROCYCLIC COMPOUNDS
IMMUNE SYSTEM DISEASES
LEUKEMIA
MAMMALS
MICE
MICROWAVE RADIATION
MUTAGENESIS
MUTAGENS
MUTATION FREQUENCY
MUTATIONS
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROFLAVINE
PYRIDINES
RADIATIONS
RODENTS
SISTER CHROMATID EXCHANGES
SYNERGISM
TUMOR CELLS
VERTEBRATES
XENOBIOTICS