Pharmacological and biochemical properties of the benzodiazepine-GABA receptor in codfish brain in comparison with mammalian brain
Thesis/Dissertation
·
OSTI ID:6159509
The GABA receptor of codfish brain is encoded by an ancestral gene of the mammalian GABA receptor based on phylogenetic studies. The mammalian GABA receptor consists of at least two subunits ({beta} and {alpha}) which could be photoaffinity labeled by the GABA agonist ({sup 3}H)muscimol (57 kDa) and the benzodiazepine (BZ) agonist ({sup 3}H)flunitrazepam (52 kDa), respectively. In contrast, electrophoresis of codfish GABA receptor photoaffinity labeled by the same ligands showed a single radioactive peak on sodium dodecyl surface polyarcylamide gel, giving rise to a relative molecular weight of 56-57 kDa equivalent to the {beta} subunit of 57 kDa in mammals. The homogeneity of purified receptor using benzodiazepine (Ro 7-1986/1) affinity chromatography was further verified by two-dimensional gel electrophoresis based on isoelectric point and molecular weight, in addition to a single band on a silver stained gel and specific activity. The receptor density and affinity constant for ({sup 3}H)muscimol and ({sup 3}H)flunitrazepam are comparable to those in bovine, rate, and human brain.
- Research Organization:
- California Univ., Los Angeles, CA (USA)
- OSTI ID:
- 6159509
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMINO ACIDS
AMINOBUTYRIC ACID
ANIMALS
AQUATIC ORGANISMS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARBOXYLIC ACIDS
CATTLE
CENTRAL NERVOUS SYSTEM
CHEMICAL COMPOSITION
CHROMATOGRAPHY
CODFISH
COMPARATIVE EVALUATIONS
DOMESTIC ANIMALS
DRUGS
ELECTROPHORESIS
FISHES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIGANDS
MAMMALS
MAN
MEMBRANE PROTEINS
MOLECULAR WEIGHT
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
RUMINANTS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TWO-DIMENSIONAL ELECTROPHORESIS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMINO ACIDS
AMINOBUTYRIC ACID
ANIMALS
AQUATIC ORGANISMS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARBOXYLIC ACIDS
CATTLE
CENTRAL NERVOUS SYSTEM
CHEMICAL COMPOSITION
CHROMATOGRAPHY
CODFISH
COMPARATIVE EVALUATIONS
DOMESTIC ANIMALS
DRUGS
ELECTROPHORESIS
FISHES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIGANDS
MAMMALS
MAN
MEMBRANE PROTEINS
MOLECULAR WEIGHT
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
RUMINANTS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TWO-DIMENSIONAL ELECTROPHORESIS
VERTEBRATES