Strongyloides stercoralis-infected dogs as a model for human disseminated strongyloidiasis

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1989

OSTI ID:6157653

Aikens, L M

The route of migration of Strongyloides stercoralis third-stage infective larvae was explored in primary and autogenous infections in the dog. Larvae was radiolabeled by one of two means: (1) by culture of the free-living L3 stage in a nutrient medium, deficient in methionine, supplemented with ({sup 75}Se)Selenomethionine, and (2) by feeding of ({sup 75}Se)Selenomethionine-labeled bacteria to microbiverous L1 and L2 stages. Third-stage labeled larvae were then injected into 10-day-old pups either subcutaneously, to study primary migration, or into the distal ileum, to study autogenous migration. At intervals after infection pups were killed and whole body compressed organ autoradiography done on individual tissues to determine organ-specific larval transit sites. Autoradiographic recoveries were analyzed in the context of a series of mathematical models designed to test migratory route hypotheses. Postulated routes of migration for primary infections included (1) the Null Hypothesis or Scramble Route in which larvae migrate to the intestines by any available route, (2) the Classical Pulmonary Route in which larvae migrate sequentially from skin, to blood, to lungs, to the trachea, esophagus and intestines, and (3) the Head Migration Route in which larvae move from caudal to cranial sites within the skin and muscle before entering the intestines. Postulated routes for autoinfective migration reiterated 1 and 2 above. Least squares comparisons, of calculated models to observed autoradiographic distributions, led us to conclude that there was no reason to reject the simplest assumption that larvae move by any available route to the definitive site in both forms of migration. Sampling through tracheostomy sites in 14 pups for larval migrants confirmed this conclusion.

• Research Organization: Pennsylvania Univ., Philadelphia, PA (USA)
• OSTI ID: 6157653
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
AUTORADIOGRAPHY
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL MODELS
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DOGS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOTOPES
LARVAE
LIPOTROPIC FACTORS
MAMMALS
MATHEMATICAL MODELS
METHIONINE
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PARASITES
PARASITIC DISEASES
PATHOGENESIS
RADIOISOTOPES
SELENIUM 75
SELENIUM ISOTOPES
VERTEBRATES