Radiolabeling and autoradiographic tracing of Toxocara canis larvae in male mice

Journal Article · Sun Feb 01 04:00:00 EST 1987 · J. Parasitol.; (United States)

DOI:https://doi.org/10.2307/3282355· OSTI ID:6498931

Wade, S E; Georgi, J R

Artificially hatched infective larvae of Toxocara canis were labeled with /sup 75/Se in Medium 199 (Gibco) containing /sup 75/Se-methionine. Male CD-1 mice were infected with radiolabeled larvae by intragastric intubation or by intraperitoneal injection. At intervals of 3-56 days mice were killed and the organs prepared for compressed organ autoradiography. Radioactivity of parasitic larvae showed an exponential decrease with time, reflecting catabolism of label with a biological half life of 26 days (effective half life of 21 days) making possible experiments lasting several months. Total body larva counts, estimated by total body autoradiography, displayed an overall downward trend, but the rate of reduction was probably not constant because no significant positive or negative trends were noted from day 14 onward in the numbers of larvae. The carcass accumulated the greatest number of larvae followed by the central nervous system, liver, and lung in that order. When the numbers of larvae were considered in relationship to the mass of tissue, there were 4 groupings: central nervous system, liver, lung, carcass, and kidney, and genito-urinary organ, pelt, and intestine. No significant difference between intragastric and intraperitoneal administration was observed in the larval distribution after the larvae had left the initial site of deposition.

• Research Organization: Cornell Univ., Ithaca
• OSTI ID: 6498931
• Journal Information: J. Parasitol.; (United States), Journal Name: J. Parasitol.; (United States) Vol. 1; ISSN JOPAA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
ASCARIDAE
ASCHELMINTHES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOLOGICAL HALF-LIFE
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
GLANDS
HELMINTHS
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOTOPES
KIDNEYS
LABELLING
LARVAE
LIPOTROPIC FACTORS
LIVER
LUNGS
MAMMALS
METHIONINE
MICE
NEMATODES
NERVOUS SYSTEM
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PARASITES
PARASITIC DISEASES
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SELENIUM 75
SELENIUM ISOTOPES
TISSUE DISTRIBUTION
VERTEBRATES