Radiolabeling of infective third-stage larvae of Strongyloides stercoralis by feeding ( sup 75 Se)selenomethionine-labeled Escherichia coli to first- and second-stage larvae

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · Journal of Parasitology; (USA)

DOI:https://doi.org/10.2307/3283058· OSTI ID:5156994

Aikens, L M; Schad, G A ^[1]

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1. Univ. of Pennsylvania, Philadelphia (USA)

A technique is described for radiolabeling Strongyloides stercoralis larvae with ({sup 75}Se)selenomethionine. Cultures of an auxotrophic methionine-dependent stain of Escherichia coli were grown in a medium containing Dulbecco's modified Eagle's medium supplemented with 5% nutrient broth, amino acids, and ({sup 75}Se)selenomethionine. When the {sup 75}Se-labeled bacterial populations were in the stationary phase of growth, cultures were harvested and the bacteria dispersed on agar plates to serve as food for S. stercoralis larvae. Use of nondividing bacteria is important for successful labeling because the isotope is not diluted by cell division and death of larvae attributable to overgrowth by bacteria is prevented. First-stage S. stercoralis larvae were recovered from feces of infected dogs and reared in humid air at 30 C on agar plates seeded with bacteria. After 7 days, infective third-stage larvae were harvested. The mean specific activity of 6 different batches of larvae ranged from 75 to 330 counts per min/larva with 91.8 +/- 9.5% of the population labeled sufficiently to produce an autoradiographic focus during a practicable, 6-wk period of exposure. Labeled infective larvae penetrated the skin of 10-day-old puppies and migrated to the small intestine, where the developed to adulthood.

• OSTI ID: 5156994
• Journal Information: Journal of Parasitology; (USA), Journal Name: Journal of Parasitology; (USA) Vol. 75:5; ISSN 0022-3395; ISSN JOPAA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ASCHELMINTHES
AUTORADIOGRAPHY
BACTERIA
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DOGS
ELECTRON CAPTURE RADIOISOTOPES
ESCHERICHIA COLI
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
HELMINTHS
HOOKWORM
INFECTIOUS DISEASES
INFECTIVITY
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOTOPES
LABELLING
LARVAE
MAMMALS
MICROORGANISMS
NEMATODES
NUCLEI
ORGANS
PARASITES
PARASITIC DISEASES
RADIOISOTOPES
SELENIUM 75
SELENIUM ISOTOPES
SMALL INTESTINE
VERTEBRATES