Anti-idiotypic antibodies that protect cells against the action of diphtheria toxin
An anti-idiotypic serum prepared against the combining site (idiotype) of specific anti-diphtheria toxoid antibodies was characterized with respect to its interaction with highly diphtheria toxin-sensitive Vero cells. Although the anti-idiotypic serum protected Vero cells against the cytotoxic action of diphtheria toxin, it did not prevent the binding of /sup 125/I-labeled diphtheria toxin to the cells but did inhibit the internalization and degradation of /sup 125/I-labeled toxin. This anti-idiotypic serum immunoprecipitated a cell-surface protein from radiolabeled Vero cells with an apparent Mr of approximately 15,000. These results are consistent with the hypothesis that the anti-idiotypic serum contains antibodies that carry an internal image of an internalization site on the toxin and that a cell-surface protein involved in toxin internalization possesses a complementary site recognized by both the toxin and the anti-idiotypic antibodies.
- Research Organization:
- Univ. of Texas Southwestern Medical Center, Dallas (USA)
- OSTI ID:
- 6155565
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 86:6
- Country of Publication:
- United States
- Language:
- English
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ANTIBODIES
BIOLOGICAL FUNCTIONS
TOXINS
BIOCHEMICAL REACTION KINETICS
CHEMICAL COMPOSITION
ELECTROPHORESIS
IMMUNOGLOBULINS
IODINE 125
MOLECULAR WEIGHT
RADIOIMMUNOASSAY
RECEPTORS
SURVIVAL TIME
ANTIGENS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
GLOBULINS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MATERIALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
REACTION KINETICS
TOXIC MATERIALS
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques