Mutant with diphtheria toxin receptor and acidification function but defective in entry of toxin

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(87)90092-9· OSTI ID:6528367

Kohno, Kenji ^[1]; Hayes, H; Mekada, Eisuke ^[2]; Uchida, Tsuyoshi ^[3]

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1. National Institute for Basic Biology, Aichi (Japan)
2. Osaka Univ. (Japan)
3. National Institute for Basic Biology, Aichi (Japan) Osaka Univ. (Japan)

A mutant of Chinese hamster ovary cells, GE1, that is highly resistant to diphtheria toxin was isolated. The mutant contains 50% ADP-ribosylatable elongation factor 2, but its protein synthesis was not inhibited by the toxin even at concentrations above 100 {mu}g/ml. {sup 125}I-labeled diphtheria toxin was associated with GE1 cells as well as with the parent cells but did not block protein synthesis of GE1 cells even when the cells were exposed to low pH in the presence or absence of NH{sub 4}Cl. The infections of GE1 cells and the parent cells by vesicular stomatitis virus were similar. GE1 cells were cross-resistant to Pseudomonas aeruginosa exotoxin A and so were about 1,000 times more resistant to this toxin than the parent cells. Hybrids of GE1 cells and the parent cells or mutant cells lacking a functional receptor were more sensitive to diphtheria toxin than GE1 cells. These results suggest that entry of diphtheria toxin into cells requires a cellular factor(s) in addition to those involved in receptor function and acidification of endosomes and that GE1 cells do not express this cellular factor. This character is recessive in GE1 cells.

• OSTI ID: 6528367
• Journal Information: Experimental Cell Research; (USA), Journal Name: Experimental Cell Research; (USA) Vol. 172:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACIDIFICATION
ANIMAL CELLS
ANTIGENS
BACTERIA
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
CHEMISTRY
CHO CELLS
DAYS LIVING RADIOISOTOPES
DIPHTHERIA
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MATERIALS
MEMBRANE PROTEINS
MICROORGANISMS
MUTANTS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
PSEUDOMONAS
RADIOISOTOPES
RECEPTORS
TOXIC MATERIALS
TOXINS
UPTAKE