Survival of mammary epithelial cells from virgin female BALB/c mice following in vivo gamma irradiation

Journal Article · Thu Nov 01 04:00:00 EST 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576347· OSTI ID:6152284

Adams, L M; Ethier, S P; Ullrich, R L

To examine the cytotoxic effects of ionizing radiation on mouse mammary epithelial cells, a transplantation assay was used to generate a single-dose survival curve. In these experiments, virgin female BALB/c mice were irradiated with 0, 100, 300, 600, 900, 1200, or 1500 rad whole-body /sup 137/Cs radiation. Mammary glands of control and irradiated mice were removed and the mammary epithelial cells were enzymatically dissociated, either immediately or 24 hr following irradiation, serially diluted, and injected into gland-free mammary fat pads of 3 1/2-week-old virgin female BALB/c host mice. The number of cells required to give ductal growth at 10 weeks in half the fat pads (the mammary cell dose 50%, MCD/sub 50/) was determined by a maximum likelihood procedure and the ratio of MCD/sub 50/ for nonirradiated control cells to MCD/sub 50/ for irradiated cells provided an estimate of cell survival. Fitting the data using a single hit multitarget model resulted in an estimate for D/sub 0/ of 250 rad with an n of 2.4. No significant difference in the results was obtained whether the cells were dissociated and transplanted immediately after irradiation or the cells were allowed to remain in situ for 24 hr following irradiation. Thus no in situ repair was detected for mouse mammary epithelial cells.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6152284
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 100:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560151* -- Radiation Effects on Animals-- Man
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL KILLING
CESIUM 137
CESIUM ISOTOPES
DATA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EPITHELIUM
EXPERIMENTAL DATA
EXTERNAL IRRADIATION
GAMMA RADIATION
GLANDS
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOTOPES
MAMMALS
MAMMARY GLANDS
MICE
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RECOVERY
REPAIR
RODENTS
SURVIVAL CURVES
TISSUES
TRANSPLANTS
VERTEBRATES
WHOLE-BODY IRRADIATION
YEARS LIVING RADIOISOTOPES