Rate of progression of radiation-transformed mammary epithelial cells is enhanced after low-dose-rate neutron irradiation

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576726· OSTI ID:5326186

Ullrich, R L

Studies in this laboratory have shown enhancement of the mammary tumorigenic effects of neutron irradiation after low-dose-rate neutron exposures. To investigate possible reasons, a mammary cell system was used which allows quantitation of initiated mammary epithelial cells and examination of the progression of these radiation-altered cells toward the neoplastic phenotype. Female BALB/c mice were irradiated with fission-spectrum neutrons at dose rates of 1 rad/min or 1 rad/day. Twenty-four hours or 16 weeks after irradiation, mammary cells were obtained by enzymatic dissociation. Mammary outgrowths were derived by injection of 10(4) cells into gland-free fat pads of 3-week-old female BALB/c mice. The frequency of ductal dysplasias in outgrowths from cells irradiated at high or low dose rates was similar. Persistence of dysplasias differed markedly. Few of the dysplasias in outgrowths derived from cells irradiated at the high dose rate persisted, while a large fraction of the dysplasias in outgrowths derived from cells irradiated at low dose rate persisted. When cells remained in situ for 16 weeks prior to dissociation a higher frequency of persistent altered cells was also observed in outgrowths derived from cells irradiated at low neutron dose rates. These data suggest that low-dose-rate neutron exposures enhance the probability of progression of carcinogen-altered cells rather than increase the numbers of initiated cells.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 5326186
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL TRANSFORMATIONS
DOSE RATES
DOSE-RESPONSE RELATIONSHIPS
ELEMENTARY PARTICLES
EPITHELIUM
FERMIONS
GLANDS
HADRONS
IRRADIATION
LOW DOSE IRRADIATION
MAMMALS
MAMMARY GLANDS
MICE
NEUTRONS
NUCLEONS
ONCOGENIC TRANSFORMATIONS
ORGANS
RADIATION EFFECTS
RODENTS
TISSUES
VERTEBRATES