Detection of ductal dysplasia in mammary outgrowths derived from carcinogen-treated virgin female BALB/c mice

Journal Article · Sat May 01 04:00:00 EDT 1982 · Cancer Res.; (United States)

OSTI ID:6842823

Ethier, S P; Ullrich, R L

These studies were undertaken to determine if altered growth potential of mammary epithelial cells could be detected in outgrowths derived from monodispersed mammary cells of virgin female BALB/c mice previously exposed to ionizing radiation or 7, 12-dimethylbenz(a)anthracene (DMBA). Twenty-four hr prior to cell dissociation, donor animals were exposed to either 100 rads of gamma-ray irradiation, 0.25 mg of DMBA, or 0.075 mg of DMBA. Control donors were untreated. Mammary outgrowths were then derived from these donor cells by injecting either 10(5) or 10(4) cells into the gland-free mammary fat pads of three-week-old virgin female BALB/c mice. Mammary outgrowths were classified either as having a normal ductal architecture or as having ductal dysplasia. Ductal dysplasias were further classified on the basis of an index of severity, which was an arbitrary index based on the number of abnormal ductal structures within each lesion. The data indicated that treatment of donor animals with either gamma-radiation or DMBA increased the frequency of ductal lesions over control levels; however, both the frequency and severity of the lesions depended on the number of cells which were injected into the fat pad. The data indicated that ductal dysplasias were more common and more severe in outgrowths derived 10(4) rather than 10(5) cells. The ductal lesions observed in this study resembled both morphologically and histologically ductal abnormalities which have been associated with the pathogenesis of mammary carcinoma in both rats and mice.

• Research Organization: University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences
• OSTI ID: 6842823
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 42:5; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
550900* -- Pathology
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
COMPARATIVE EVALUATIONS
CONDENSED AROMATICS
DIMETHYLBENZANTHRACENE
DISEASES
ELECTROMAGNETIC RADIATION
EPITHELIUM
GAMMA RADIATION
GLANDS
HISTOLOGY
IONIZING RADIATIONS
MAMMALS
MAMMARY GLANDS
MICE
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGY
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RESEARCH PROGRAMS
RODENTS
TISSUES
VERTEBRATES