Nuclear Medicine Program progress report for quarter ending September 30, 1988

Technical Report · Wed Mar 01 04:00:00 EST 1989

DOI:https://doi.org/10.2172/6151410· OSTI ID:6151410

Knapp, Jr., F. F.; Ambrose, K. R.; Callahan, A. P.; McPherson, D. W.; Srivastava, P. C.; Allred, J. F.; Blystone, S. L.; Kropp, A.; Lisic, E. C.; Rice, D. E.

During this period the properties of the unknown metabolite released from Langendorff-perfused rat hearts administered radioiodinated 15-(p-iodophenyl)-3-R,S-methylpentadecanoic acid (BMIPP) have been further evaluated. Identification of this metabolite is important to provide a better understanding of the myocardial metabolism of methyl-branched fatty acids and to illuminate the factors affecting myocardial retention of such agents. The metabolite is the principal component in the outflow of the isolated rat hearts. Following isolation and purification of the metabolite, treatment with NaBH/sub 4/ had no effect on the chromatographic properties. In contrast, a much less polar product was formed by treatment with acetic anhydride, suggesting the presence of a primary or secondary hydroxyl group. In addition, the metabolite is soluble in dilute base and extracted from an acid solution with ether, demonstrating the presence of a carboxyl group. These combined results suggest BMIPP is metabolized to a hydroxy acid of unknown structure. Studies are now in progress to identify this material. Studies of the effects of chain length on the complexation of a series of p-carboxyalkylphenylglyoxal bis-(N-alkylthiosemicarbazones) (TSC) have continued. After complexation with either Cu-64 or Cu-67 followed by activation to the tetrafluorophenyl esters, the bifunctional ligands were attached to BSA and purified by G-25 Sephadex. Yields varied from 2--3% to 40%, with higher yields for the shorter chain analogues. Because of simpler formation and higher yield, future studies will focus on the radiolabeling of antibodies with the short-chain analogues of the 1,2-diketone TSC derivatives. Also during this period (I-131)IPPA was supplied to collaborators at the Institute of Clinical and Experimental Nuclear Medicine in Bonn, West Germany, for studies with an isolated working rat heart model.

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6151410
• Report Number(s): ORNL/TM-11043; ON: DE89009685
• Country of Publication: United States
• Language: English

Related Subjects

550500 -- Metabolism
550600* -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGY
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHELATING AGENTS
CHEMICAL REACTIONS
CHEMISTRY
COPPER 64
COPPER 67
COPPER ISOTOPES
DAYS LIVING RADIOISOTOPES
DOCUMENT TYPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HALOGENATION
HEART
HEAVY NUCLEI
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINATION
IRIDIUM 192
IRIDIUM ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MEDICINE
METABOLITES
MINUTES LIVING RADIOISOTOPES
MONOCARBOXYLIC ACIDS
MONOCLONAL ANTIBODIES
NATIONAL ORGANIZATIONS
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
ORNL
PROGRESS REPORT
PURIFICATION
RADIOBIOLOGY
RADIOCHEMISTRY
RADIOISOTOPES
RADIOPHARMACEUTICALS
RATS
RHENIUM 186
RHENIUM 188
RHENIUM ISOTOPES
RODENTS
SALES
SYNTHESIS
US AEC
US DOE
US ERDA
US ORGANIZATIONS
VERTEBRATES
YEARS LIVING RADIOISOTOPES