Changes in the viscosity of hyaluronic acid after exposure to a myeloperoxidase-derived oxidant
Journal Article
·
· Arthritis Rheum.; (United States)
Both purified hyaluronic acid (HA) and bovine synovial fluid react with OCI-, the major oxidant produced by the myeloperoxidase (MPO)/H/sub 2/O/sub 2//CI- system, resulting in a decrease in their specific viscosity. This reaction is inhibited in the presence of excess methionine. H/sub 2/O/sub 2/ alone decreases the viscosity of HA, presumably by the Fenton reaction, in the absence (but not in the presence) of the iron chelator, diethyltriaminepentacetic acid (DETAPAC). In the presence of DETAPAC, incubation of HA with the complete MPO/H/sub 2/O/sub 2//CI- system lowered the viscosity of HA. Analysis of 3H-HA exposed to OCI- by gel filtration chromatography indicated that cleavage of HA occurred only at higher OCI- concentrations. We suggest that the reduction in viscosity of HA by the MPO/H/sub 2/O/sub 2//CI- system may be due to a combination of oxidative cleavage and changes in the conformation of the molecule. We speculate that the changes in the molecular size of rheumatoid synovial fluid HA may be due to the action of the neutrophil MPO/H/sub 2/O/sub 2//CI- system.
- Research Organization:
- Australian National Univ., Canberra
- OSTI ID:
- 6146377
- Journal Information:
- Arthritis Rheum.; (United States), Journal Name: Arthritis Rheum.; (United States) Vol. 32:4; ISSN ARHEA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AMINO ACIDS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARBOXYLIC ACIDS
CHELATING AGENTS
DRUGS
HYALURONIC ACID
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
METHIONINE
MUCOPOLYSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
PHOTOCHEMICAL OXIDANTS
POLYSACCHARIDES
SACCHARIDES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VISCOSITY
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AMINO ACIDS
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARBOXYLIC ACIDS
CHELATING AGENTS
DRUGS
HYALURONIC ACID
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
METHIONINE
MUCOPOLYSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
PHOTOCHEMICAL OXIDANTS
POLYSACCHARIDES
SACCHARIDES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VISCOSITY