Effect of deuterium oxide on neutrophil oxidative metabolism, phagocytosis, and lysosomal enzyme release

Journal Article · Wed Dec 01 04:00:00 EST 1982 · Inflammation (N.Y.); (United States)

DOI:https://doi.org/10.1007/BF00917309· OSTI ID:5993283

Tsan, M F; Turkall, R M

We have previously shown that deuterium oxide (D/sub 2/O) enhances the oxidation of methionine, a myeloperoxidase (MPO) -mediated reaction, by human neutrophils during phagocytosis. However, D/sub 2/O has no effect on the oxidation of methionine by the purified MPO-H/sub 2/O/sub 2/-Cl- system. To explain this observation, we studied the effect of D/sub 2/O on the oxidative metabolism, phagocytosis, and lysosomal enzyme release by human neutrophils. D/sub 2/O stimulated the hexose monophosphate shunt (HMS) activity of resting neutrophils in a dose-response fashion. In the presence of latex particles or phorbol myristate acetate (PMA), D/sub 2/O brought about an exaggerated stimulation of the HMS activity. This enhancement of the HMS activity by D/sub 2/O was markedly reduced when neutrophils form two patients with X-linked chronic granulomatous disease (CGD) were used, either in the presence or absence of latex particles or PMA. Superoxide and H/sub 2/O/sub 2/ production by neutrophils in the presence of latex particles or PMA were also stimulated by D/sub 2/O. In contrast, D/sub 2/O inhibited the ingestion of latex particles. D/sub 2/O enhanced the extracellular release of MPO, but not lactate dehydrogenase, by neutrophils only in the simultaneous presence of cytochalasin B and latex particles. The enhancement of HMS activity and MPO release by D/sub 2/O was partially inhibited by colchicine. Our results suggest that enhancement of neutrophil oxidative metabolism by D/sub 2/O may in part explain the stimulation of methionine oxidation by phagocytosing neutrophils.

• Research Organization: Division of Hematology, Johns Hopkins Medical Institutions, Baltimore, Maryland
• OSTI ID: 5993283
• Journal Information: Inflammation (N.Y.); (United States), Journal Name: Inflammation (N.Y.); (United States) Vol. 6:4; ISSN INFLD
• Country of Publication: United States
• Language: English

Related Subjects

560122* -- Radiation Effects on Cells-- Internal Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENZYME ACTIVITY
HEAVY WATER
HYDROGEN COMPOUNDS
LEUKOCYTES
LIPOTROPIC FACTORS
MATERIALS
METHIONINE
NEUTROPHILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PHAGOCYTOSIS
TOXICITY
WATER