Capped poly(A) leaders of variable lengths at the 5' ends of vaccinia virus late mRNAs

Journal Article · Sun Jan 01 04:00:00 EST 1989 · J. Virol.; (United States)

OSTI ID:6143961

Ahn, B Y; Moss, B

Evidence for capped poly(A) leaders of variable lengths located immediately upstream of the translation initiation codon was obtained by direct analyses of a major late mRNA species. A decapping-recapping method was used to specifically substitute a radioactively labeled phosphate for an unlabeled one within the cap structure. RNase H-susceptible sites were made by hybridizing synthetic oligodeoxyribonucleotides to the mRNA encoding a late major structural protein of 11 kilodaltons. Sequences of the type m/sup 7/G(5')pppAmp (Ap)/sub n/UpG /hor elipsis/, where n varies from a few to more than 40 nucleotides, were deduced by analysis of the length and sequence of RNase, H, RNase T/sub 1/, and RNase U2 digestion products.

• Research Organization: National Institute of Allergy and Infectious Diseases, Bethesda, MD (USA)
• OSTI ID: 6143961
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 63:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CODONS
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERASES
GENE REGULATION
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MESSENGER-RNA
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RNA
RNA-ASE
VACCINIA VIRUS
VIRUSES