Proteins bound to polyribosomal mRNA of the embryonic axis of kidney bean seeds
Journal Article
·
· Biochemistry (Engl. Transl.); (United States)
OSTI ID:5767807
The protein composition of polyribosome-bound mRNAs from the embryonic axis of germinating kidney bean seeds, isolated by an improved procedure including treatment with EDTA, low concentrations of RNase T/sub 1/, and centrifugation in a linear 15-40% sucrose concentration gradient, was investigated. Electrophoretic analysis of the proteins of purified fragments of mRNP obtained by the method described showed the presence of two to three major polypeptides (molecular weights 50, 38, 32 kilodaltons) and a number of minor ones. It was established that one of the major proteins is associated with the poly(A) sequences of mRNA (50 kilodaltons). The similarity of the protein compositions of polyribosomal mRNAs of kidney bean embryos and other objects studied is discussed.
- Research Organization:
- N.G. Kholodnyi Institute of Biochemistry, Kiev, USSR
- OSTI ID:
- 5767807
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 51:8; ISSN BIORA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMAL CELLS
AZINES
BEANS
BINDING ENERGY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CENTRIFUGATION
CHELATING AGENTS
CHEMICAL ANALYSIS
EDTA
ELECTROPHORESIS
EMBRYONIC CELLS
ENERGY
ENZYMES
ESTERASES
FOOD
GERMINATION
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
LABELLING
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PEPTIDES
PHOSPHODIESTERASES
PLANTS
POLYPEPTIDES
PROTEINS
PYRIMIDINES
QUANTITATIVE CHEMICAL ANALYSIS
RADIOACTIVITY
RADIOASSAY
RIBOSIDES
RIBOSOMES
RNA
RNA-ASE
SEEDS
SEPARATION PROCESSES
TRITIUM COMPOUNDS
URACILS
URIDINE
VEGETABLES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMAL CELLS
AZINES
BEANS
BINDING ENERGY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CENTRIFUGATION
CHELATING AGENTS
CHEMICAL ANALYSIS
EDTA
ELECTROPHORESIS
EMBRYONIC CELLS
ENERGY
ENZYMES
ESTERASES
FOOD
GERMINATION
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
LABELLING
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PEPTIDES
PHOSPHODIESTERASES
PLANTS
POLYPEPTIDES
PROTEINS
PYRIMIDINES
QUANTITATIVE CHEMICAL ANALYSIS
RADIOACTIVITY
RADIOASSAY
RIBOSIDES
RIBOSOMES
RNA
RNA-ASE
SEEDS
SEPARATION PROCESSES
TRITIUM COMPOUNDS
URACILS
URIDINE
VEGETABLES