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Studies on the amount of single-stranded DNA present in Chinese hamster ovary cells during the repair of damage induced by x rays of methyl methanesulfonate

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3575531· OSTI ID:6142123
;

Benzoylated naphthoylated DEAE-cellulose chromatography was used to determine the amount of single-stranded DNA present in Chinese hamster ovary cells after x irradiation or 1 mM methyl methanesulfonate (MMS) treatment for 1 h at 37/sup 0/C. The MMS treatment resulted in a 1.5- to 2.5-fold greater amount of single-stranded DNA than controls when analyzed 120 min after treatment. Although no significant difference (P = 0.05) in the amount of single-stranded DNA was found between control and irradiated (30 Gy) cells when repair times of 30 and 120 min were examined, the amount of single-stranded DNA increased by 30 to 35% when cells were irradiated (30 to 200 Gy) and examined after a 60-min repair time. Furthermore, this increase in single-stranded DNA was observed only when the DNA was sheared under low-salt conditions. These findings and the presence of small molecules of about 1 x 10/sup 5/ daltons in alkaline sucrose gradients of DNA isolated from irradiated cells suggest that regions ofof this damage during repair time.

Research Organization:
Colorado State Univ., Fort Collins
OSTI ID:
6142123
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 87:3; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL CULTURES
CHEMISTRY
COMPARATIVE EVALUATIONS
DNA
ELECTROMAGNETIC RADIATION
ESTERS
FEMALE GENITALS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GONADS
HAMSTERS
IONIZING RADIATIONS
MAMMALS
METHYL METHANESULFONATE
MOLECULAR BIOLOGY
MUTAGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OVARIES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
SULFONIC ACID ESTERS
TIME DEPENDENCE
VERTEBRATES
X RADIATION