Differences in detection of DNA adducts in the sup 32 P-postlabelling assay after either 1-butanol extraction or nuclease Pl treatment
Technical Report
·
OSTI ID:6140928
The use of nuclease P1 treatment and 1-butanol extraction to increase the sensitivity of the {sup 32}P-postlabelling assay for DNA adducts have been compared. Although similar results were obtained with the two methods for standard adducts formed with benzo(a)pyrene diol epoxide I, nuclease P1 treatment resulted in a significant reduction in detection of major adducts 1-amino-6-nitropyrene, 1-amino-8-nitropyrene, 2-aminofluorene, 2-naphthylamine and 4-aminobiphenyl modified DNAs, but not following the {sup 32}P-postlabelling analysis of 2-acetylaminofluorene modified DNA. These results suggest that at least initially, both modications of the {sup 32}P-postlabelling assay should be used for the detection of unknown adducts or for adducts derived from nitro-aromatics and aromatic amines.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
- OSTI ID:
- 6140928
- Report Number(s):
- PB-91-115972/XAB; EPA--600/J-89/471
- Country of Publication:
- United States
- Language:
- English
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· Environ. Health Perspect.; (United States)
·
OSTI ID:5068033
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OSTI ID:5837222
Related Subjects
550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLAMINOFLUORENES
ADDUCTS
ALCOHOLS
AMINES
AROMATICS
BENZOPYRENE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BUTANOLS
CONDENSED AROMATICS
DAYS LIVING RADIOISOTOPES
DNA ADDUCTS
ENZYMES
EPOXIDES
ESTERASES
EXTRACTION
HYDROCARBONS
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIGHT NUCLEI
METABOLISM
NITRO COMPOUNDS
NUCLEASES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SEPARATION PROCESSES
SOLVENT EXTRACTION
TRACER TECHNIQUES
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLAMINOFLUORENES
ADDUCTS
ALCOHOLS
AMINES
AROMATICS
BENZOPYRENE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BUTANOLS
CONDENSED AROMATICS
DAYS LIVING RADIOISOTOPES
DNA ADDUCTS
ENZYMES
EPOXIDES
ESTERASES
EXTRACTION
HYDROCARBONS
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIGHT NUCLEI
METABOLISM
NITRO COMPOUNDS
NUCLEASES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SEPARATION PROCESSES
SOLVENT EXTRACTION
TRACER TECHNIQUES