Detection and comparison of DNA adducts after in vitro and in vivo diesel emission exposures
Technical Report
·
OSTI ID:5837222
Development of methodologies to evaluate certain classes of polycyclic aromatic compounds (PAC) detected in complex mixtures to which humans are exposed would greatly improve the diagnostic potential of (32)P-postlabeling analysis. Identification of DNA adduct patterns of specific exposure-related marker adducts would strengthen associations between observed DNA adducts and exposures to different environmental pollutants (e.g., kerosene, cigarette smoke, coke oven, and diesel). Diesel-modified DNA adduct patterns were compared in various in vitro and in vivo rodent model systems and then compared to DNA reactive oxidative and reductive metabolites of 1-nitropyrene. The formation of nitrated-polycyclic aromatic hydrocarbon (nitrated-PAH) DNA adducts, derived from the metabolism of diesel extract constituents, was enhanced relative to other PAH-derived DNA adducts via xanthine oxidase-catalyzed nitroreduction. These adducts were detectable only by the butanol extraction version of the postlabeling analysis. Marker adducts detected in the various test systems presented here will assist in characterizing nuclease-P1-sensitive nitrated PAH adducts in humans.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC (United States). Health Effects Research Lab.
- OSTI ID:
- 5837222
- Report Number(s):
- PB-93-228856/XAB; EPA--600/J-93/359
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Technical Report
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Mon Dec 31 23:00:00 EST 1990
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OSTI ID:5996071
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OSTI ID:45252
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· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:6100129
Related Subjects
550400 -- Genetics
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMALS
AROMATICS
DETECTION
DIAGNOSTIC TECHNIQUES
DIESEL FUELS
DISTILLATES
DNA ADDUCTS
ENERGY SOURCES
ENVIRONMENTAL EXPOSURE
EXHAUST GASES
FLUIDS
FOSSIL FUELS
FUELS
GAS OILS
GASEOUS WASTES
GASES
HAZARDS
HEALTH HAZARDS
HUMAN POPULATIONS
HYDROCARBONS
IN VITRO
IN VIVO
LIQUID FUELS
MAMMALS
MEASURING METHODS
METABOLITES
ORGANIC COMPOUNDS
PETROLEUM
PETROLEUM DISTILLATES
PETROLEUM FRACTIONS
PETROLEUM PRODUCTS
POLYCYCLIC AROMATIC HYDROCARBONS
POPULATIONS
RODENTS
VERTEBRATES
WASTES
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMALS
AROMATICS
DETECTION
DIAGNOSTIC TECHNIQUES
DIESEL FUELS
DISTILLATES
DNA ADDUCTS
ENERGY SOURCES
ENVIRONMENTAL EXPOSURE
EXHAUST GASES
FLUIDS
FOSSIL FUELS
FUELS
GAS OILS
GASEOUS WASTES
GASES
HAZARDS
HEALTH HAZARDS
HUMAN POPULATIONS
HYDROCARBONS
IN VITRO
IN VIVO
LIQUID FUELS
MAMMALS
MEASURING METHODS
METABOLITES
ORGANIC COMPOUNDS
PETROLEUM
PETROLEUM DISTILLATES
PETROLEUM FRACTIONS
PETROLEUM PRODUCTS
POLYCYCLIC AROMATIC HYDROCARBONS
POPULATIONS
RODENTS
VERTEBRATES
WASTES