Improvement in the diagnostic potential of (32)P-postlabeling analysis demonstrated by the selective formation and comparative analysis of nitrated-PAH-derived adducts arising from diesel-particle extracts

Technical Report · Tue Jan 01 04:00:00 EST 1991

OSTI ID:5996071

Gallagher, J E; Kohan, M J; George, M H; Lewtas, J

Studies suggest that DNA adducts derived from N-substituted aryl-compounds are poorly recovered in the nuclease P1 version of the (32)P-postlabeling assay but not the butanol extraction version. Both versions were employed to ascertain whether the differences in sensitivity could be used to select for nitroaromatic-DNA adducts derived by treating calf thymus DNA with organic extracts from four diesel and one gasoline vehicle emission particles. The authors' enhanced the formation of nitrated-PAH-derived adducts through xanthine oxidase-catalyzed nitroreduction of nitrated-polycyclic aromatic hydrocarbons; constituents previously detected in the diesel emissions. All four diesel organic extracts treated with xanthine oxidase resulted in the formation of one major DNA adduct chromatographically distinct from the multiple DNA adducts detected in the rat liver S9-treated incubations. The adduct was detectable with the butanol extraction but not the nuclease P1 version of the (32)P-postlabeling assay and was chromatographically similar to DNA adducts formed following xanthine oxidase nitroreduction of 1-nitropyrene or ascorbic acid treatment of 1-nitro-8-nitrosopyrene and 1-nitro-6-nitrosopyrene.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC (United States). Health Effects Research Lab.
• OSTI ID: 5996071
• Report Number(s): PB-92-110485/XAB
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CATTLE
DAYS LIVING RADIOISOTOPES
DIESEL FUELS
DNA ADDUCTS
DOMESTIC ANIMALS
ENERGY SOURCES
ENZYMES
EXTRACTION
FOSSIL FUELS
FUELS
GAS OILS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIQUID FUELS
MAMMALS
METABOLIC ACTIVATION
NITRO COMPOUNDS
NITROSO COMPOUNDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PETROLEUM
PETROLEUM FRACTIONS
PETROLEUM PRODUCTS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RATS
RODENTS
RUMINANTS
SEPARATION PROCESSES
SOLVENT EXTRACTION
SYNTHESIS
TOXICITY
TRACER TECHNIQUES
VERTEBRATES