Methanogenic cleavage of acetate by lysates of Methanosarcina barkeri
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:6134458
Cell lysates of acetate-grown Methanosarcina barkeri 227 were found to cleave acetate to CH/sub 4/ and CO/sub 2/. The aceticlastic reaction was identified by using radioactive methyl-labeled acetate. Cell lysates decarboxylated acetate in a nitrogen atmosphere, conserving the methyl group in methane. The rate of methanogenesis from acetate in the cell lysates was comparable to that observed with whole cells. Aceticlastic activity was found in the particulate fraction separate from methylcoenzyme M methylreductase activity, which occurs in the soluble fraction. Pronase treatment eliminated methylcoenzyme M methylreductase activity in lysates and stimulated aceticlastic activity, indicating the aceticlastic activity was not derived from unbroken cells, which are unaffected by proteolytic treatment.
- Research Organization:
- Univ. of Illinois, Urbana
- OSTI ID:
- 6134458
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 160:1; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
090122* -- Hydrocarbon Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALKANES
BACTERIA
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACID SALTS
CHALCOGENIDES
CHEMICAL REACTIONS
COENZYMES
DECARBOXYLATION
ELEMENTS
ENZYME ACTIVITY
ENZYMES
HYDROCARBONS
LABELLING
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
NITROGEN
NONMETALS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
SYNTHESIS
090122* -- Hydrocarbon Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALKANES
BACTERIA
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACID SALTS
CHALCOGENIDES
CHEMICAL REACTIONS
COENZYMES
DECARBOXYLATION
ELEMENTS
ENZYME ACTIVITY
ENZYMES
HYDROCARBONS
LABELLING
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
NITROGEN
NONMETALS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
SYNTHESIS