Kinetics of the methanogenic fermentation of acetate. [Methanosarcina barkeri]

Journal Article · Mon Oct 01 04:00:00 EDT 1990 · Applied and Environmental Microbiology; (United States)

OSTI ID:5495796

Fukuzaki, Satoshi; Nishio, Naomichi; Nagai, Shiro ^[1]

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1. Hiroshima Univ. (Japan)

Inhibition of the fermentation of acetate to methane and carbon dioxide by acetate was analyzed with an acetate-acclimatized sludge and with Methanosarcina barkeri Fusaro under mesophilic conditions. A second-order substrate inhibition model, q{sub CH{sub 4}} = q{sub m}S/(K{sub s} + S + (S{sup 2}/K{sub i})), where S was the concentration of undissociated acetic acid, not ionized acetic acid, could be applicable in both cases. The analysis resulted in substrate saturation constants, K{sub s}, of 4.0 {mu}M for the acclimatized sludge and 104 {mu}M for M. barkeri. The threshold concentrations of undissociated acetic acid when no further acetate utilization was observed were 0.078 {mu}M (pH 7.50) for the acclimatized sludge and 4.43 {mu}M (pH 7.45) for M. barkeri. These kinetic results suggested that the concentration of undissociated acetic acid became a key factor governing the actual threshold acetate concentration for acetate utilization and that the acclimatized sludge in which Methanothrix spp. appeared dominant could utilize acetate better and survive at a lower concentration of undissociated acetic acid than could M. barkeri.

• OSTI ID: 5495796
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 56:10; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090900* -- Biomass Fuels-- Processing-- (1990-)
ACETATES
ALKANES
ANAEROBIC DIGESTION
BACTERIA
BIOCONVERSION
BIOSYNTHESIS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACID SALTS
CHALCOGENIDES
DIGESTION
FERMENTATION
HYDROCARBONS
INHIBITION
KINETICS
MANAGEMENT
MATHEMATICAL MODELS
MESOPHILIC CONDITIONS
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PROCESSING
SYNTHESIS
WASTE MANAGEMENT
WASTE PROCESSING