Localization of aldosterone and corticosterone in the central nervous system, assessed by quantitative autoradiography
Nuclear localization of tritiated aldosterone in the CNS was studied in rats by numerical evaluation of silver grains, deposited over neuronal cell nuclei in thaw-mounted autoradiograms, and compared with the localization obtained after prior administration of a 100-fold excess of radioinert aldosterone, corticosterone or 18-hydroxy-11-deoxycorticosterone (18-OH-DOC). Corticosterone and 18-OH-DOC completely prevented nuclear localization in most regions examined. However, in contrast to pretreatment with aldosterone, pretreatment with corticosterone and 18-OH-DOC did not completely prevent the concentration of radioactivity in the cell nuclei of the indusium griseum. Traces of radioactivity were, furthermore, retained in areas CA1 and CA2 and the dentate gyrus in rats exposed to corticosterone, but not to 18-OH-DOC, prior to (/sup 3/H)aldosterone. A similar profile of silver grain distribution to that noted with aldosterone was found for corticosterone except that with tritiated corticosterone the most intense concentration of radioactivity occurred in hippocampal areas CA1 and CA2 and not in the indusium griseum. The authors conclude that (1) a receptor readily shared by aldosterone, corticosterone, 18-OH-DOC and DOC, but not by dihydrotestosterone, is widely distributed throughout the CNS, (2) a receptor shared by aldosterone and 18-OH-DOC, but not by corticosterone may be present in hippocampal areas CA1 and CA2, (3) that both these as well as the receptor accepting dihydrotestosterone can be located within the same cell.
- Research Organization:
- Univ. of North Carolina, Chapel Hill
- OSTI ID:
- 6132614
- Journal Information:
- Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 9:3; ISSN NERED
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effects of stress, circadian rhythms, and dietary sodium on brain cell-nuclear uptake of aldosterone and corticosterone
Type I receptors in parotid, colon, and pituitary are aldosterone selective in vivo
Related Subjects
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL LOCALIZATION
CENTRAL NERVOUS SYSTEM
CORTICOSTEROIDS
CORTICOSTERONE
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
LABELLED COMPOUNDS
MAMMALS
MINERALOCORTICOIDS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
PREGNANES
RADIONUCLIDE KINETICS
RATS
RECEPTORS
RODENTS
STEROID HORMONES
STEROIDS
TRITIUM COMPOUNDS
VERTEBRATES