Profile of the intestinal mucosal corticosteroid receptors in the domestic duck
The corticosteroid receptor profile of the intestinal tract of the domestic duck (maintained on either a low-sodium (LS) or a high-sodium (HS) diet) was investigated. Using tritiated triamcinolone acetonide (TA), corticosterone, or aldosterone as ligands, cytoplasmic mineralocorticoid receptors (MR, type I) and glucocorticoid receptors (GR, type II) were found in the mucosal cytosol of the jejunum and colon. The diet little influenced the GR binding parameters, while the MR (aldosterone) binding parameters showed a down-regulation following LS diets. The competition hierarchy of radioinert steroids on the formation of the (TH)corticosterone-receptor complex was corticosterone = cortisol = 11-deoxycorticosterone greater than aldosterone = TA = dexamethasone much greater than 11-deoxycortisol; with (TH)aldosterone, the competition was corticosterone = progesterone = 11-deoxycorticosterone greater than aldosterone = cortisol = TA = dexamethasone greater than 11-deoxycortisol greater than 11-dehydrocorticosterone. On linear sucrose gradients, receptor-ligand complexes sedimented with a single peak at 8.5 S (hypotonic gradient) and 4.0-4.5 S (hypertonic gradient), respectively. Heat-activated (TH)TA- and (TH)aldosterone-receptor complexes bound avidly to DNA-cellulose and, upon ion-exchange chromatography on DEAE-Sephacel, the presence of the negatively charged unactivated and the more positively charged activated complexes could be shown.
- Research Organization:
- Hopital Notre-Dame, Montreal, Quebec, Canada
- OSTI ID:
- 6084355
- Journal Information:
- Gen. Comp. Endocrinol.; (United States), Journal Name: Gen. Comp. Endocrinol.; (United States) Vol. 1; ISSN GCENA
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIRDS
BODY
CORTICOSTEROIDS
CORTICOSTERONE
DIGESTIVE SYSTEM
DNA
DUCKS
ENZYMES
FOWL
GASTROINTESTINAL TRACT
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
INTESTINES
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
MEMBRANES
MINERALOCORTICOIDS
MUCOUS MEMBRANES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES