Plasma membrane reorganization induced by tumor promoters in an epithelial cell line
The effects of phorbol ester tumor promoters on the lateral diffusion in plasma membrane lipid environments were examined by the technique of fluorescence recovery after photobleaching. To this end, the probe collarein, a fluorescent lipid analog that has the property of exclusive localization in the plasma membrane, was synthesized. Measured decreases in three parameters (percentage of fluorescence bleached (30%), percentage of recovery (52%), and half-time for recovery (52%)) connoted the appearance of an immobile fraction upon exposure to tumor promoters. These data are consistent with lipid reorganization in response to a reorganization of the intra- and perimembranous macromolecular scaffolding upon the interaction of cells with tumor promoters. The idea of induced reorganization is supported by experiments in which cell shape change, brought about by either exposure to cytochalasin B or growth on matrices of collagen, fibronectin, or laminin, resulted in values in the fluorescence recovery after photobleaching technique similar to those with active phorbol esters. 35 references, 3 figures, 1 table.
- Research Organization:
- Univ. of California, Berkeley, CA (United States)
- DOE Contract Number:
- AC03-76SF00098; AT03-80ER10700
- OSTI ID:
- 6098806
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 81:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
MORPHOLOGICAL CHANGES
ONCOGENIC TRANSFORMATIONS
BIOLOGICAL MODELS
PHORBOL ESTERS
BIOLOGICAL EFFECTS
CELL CULTURES
CELL MEMBRANES
DIFFUSION
EPITHELIUM
FLUORESCENCE
LIPIDS
ANIMAL TISSUES
BODY
CARCINOGENS
CELL CONSTITUENTS
ESTERS
LUMINESCENCE
MEMBRANES
ORGANIC COMPOUNDS
TISSUES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)