skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Plasma membrane reorganization induced by chemical transformation in cultura

Abstract

Induction of increased rigidity in the plasma membrane paralleling properties associated with a transformed state was suggested by two experiments. Fluorescence recovery after photobleaching (FRAP) indicated the induction of an environment in the plasma membrane where the synthetic fluorescent phospholipid collarein was immobile on the FRAP timescale. The other technique revealed the binding of epidermal growth factor (EGF) to a cryptic class of receptors which become accessible upon chemical transformation. These two lines of evidence are consistent with a reorganization of the plasma membrane induced by tumor promoters. 110 references, 38 figures, 4 tables.

Authors:
Publication Date:
Research Org.:
Lawrence Berkeley Lab., CA (USA)
OSTI Identifier:
7192531
Report Number(s):
LBL-17712
ON: DE84012311
DOE Contract Number:
AC03-76SF00098
Resource Type:
Technical Report
Resource Relation:
Other Information: Portions are illegible in microfiche products. Thesis
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CELL MEMBRANES; BIOCHEMISTRY; BIOPHYSICS; ONCOGENIC TRANSFORMATIONS; CELL CULTURES; LIPIDS; LIPOSOMES; MASS SPECTROSCOPY; NUCLEAR MAGNETIC RESONANCE; PHORBOL ESTERS; CARCINOGENS; CELL CONSTITUENTS; CHEMISTRY; ESTERS; MAGNETIC RESONANCE; MEMBRANES; ORGANIC COMPOUNDS; ORGANOIDS; RESONANCE; SPECTROSCOPY; 550200* - Biochemistry; 560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)

Citation Formats

Packard, B.S.. Plasma membrane reorganization induced by chemical transformation in cultura. United States: N. p., 1984. Web.
Packard, B.S.. Plasma membrane reorganization induced by chemical transformation in cultura. United States.
Packard, B.S.. 1984. "Plasma membrane reorganization induced by chemical transformation in cultura". United States. doi:.
@article{osti_7192531,
title = {Plasma membrane reorganization induced by chemical transformation in cultura},
author = {Packard, B.S.},
abstractNote = {Induction of increased rigidity in the plasma membrane paralleling properties associated with a transformed state was suggested by two experiments. Fluorescence recovery after photobleaching (FRAP) indicated the induction of an environment in the plasma membrane where the synthetic fluorescent phospholipid collarein was immobile on the FRAP timescale. The other technique revealed the binding of epidermal growth factor (EGF) to a cryptic class of receptors which become accessible upon chemical transformation. These two lines of evidence are consistent with a reorganization of the plasma membrane induced by tumor promoters. 110 references, 38 figures, 4 tables.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 1984,
month = 4
}

Technical Report:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that may hold this item. Keep in mind that many technical reports are not cataloged in WorldCat.

Save / Share:
  • The overall objective is to develop an initial chemical and structural basis for understanding UF 4 hydrolysis in the environment.
  • The effects of phorbol ester tumor promoters on the lateral diffusion in plasma membrane lipid environments were examined by the technique of fluorescence recovery after photobleaching. To this end, the probe collarein, a fluorescent lipid analog that has the property of exclusive localization in the plasma membrane, was synthesized. Measured decreases in three parameters [percentage of fluorescence bleached (30%), percentage of recovery (52%), and half-time for recovery (52%)] connoted the appearance of an immobile fraction upon exposure to tumor promoters. These data are consistent with lipid reorganization in response to a reorganization of the intra- and perimembranous macromolecular scaffolding uponmore » the interaction of cells with tumor promoters. The idea of induced reorganization is supported by experiments in which cell shape change, brought about by either exposure to cytochalasin B or growth on matrices of collagen, fibronectin, or laminin, resulted in values in the fluorescence recovery after photobleaching technique similar to those with active phorbol esters.« less
  • The effects of phorbol ester tumor promoters on the lateral diffusion in plasma membrane lipid environments were examined by the technique of fluorescence recovery after photobleaching. To this end, the probe collarein, a fluorescent lipid analog that has the property of exclusive localization in the plasma membrane, was synthesized. Measured decreases in three parameters (percentage of fluorescence bleached (30%), percentage of recovery (52%), and half-time for recovery (52%)) connoted the appearance of an immobile fraction upon exposure to tumor promoters. These data are consistent with lipid reorganization in response to a reorganization of the intra- and perimembranous macromolecular scaffolding uponmore » the interaction of cells with tumor promoters. The idea of induced reorganization is supported by experiments in which cell shape change, brought about by either exposure to cytochalasin B or growth on matrices of collagen, fibronectin, or laminin, resulted in values in the fluorescence recovery after photobleaching technique similar to those with active phorbol esters. 35 references, 3 figures, 1 table.« less