Micronuclei in mouse skin cells following in vivo exposure to benzo(a)pyrene, 7,12-dimethylbenz(a)anthracene, chrysene, pyrene and urethane

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Environmental and Molecular Mutagenesis; (United States)

OSTI ID:6097864

He, Shuilin ^[1]; Baker, R ^[2]

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1. Guangxi Medical Coll., Nanning (China)
2. National Inst. of Occupational Health and Safety, Sydney, New South Wales (Australia)

Detection of micronuclei (MN) in skin cells from HRA/Skh hairless mice treated with chemical or physical agents may prove informative in qualitative and quantitative studies of skin carcinogenesis. MN induction and cell survival were estimated in cytokinesis-blocked keratinocytes, cultured for 4 days in vitro, after a single topical dose of various organic compounds. Treatment with 7,12-dimethylbenz(a)anthracene (DMBA) resulted in maximal MN induction in cells removed from skin 12-24 hr after topical administration. Even in cells removed only 1 hr after DMBA treatment, a significant increase in MN was evident. However, to allow sufficient time for metabolic activation, a sampling time of 24 hr was adopted for all test substances. Dose-dependent increases in MN were observed with DMBA, benzo(a)pyrene, chrysene, and urethane. Increased numbers of micronucleated cells were detected at the lowest doses administered in the present study. Although reduced cell recovery occurred following exposure of mice to acetone, pyrene, and other chemicals, there was no evidence that cytotoxicity contributed to MN scored in keratinocytes. Moreover, the probable noncarcinogen, pyrene, failed to induce MN at doses from 2.5 {mu}g to 2.5 mg/mouse. These results show that it is possible to assess chemical exposure in skin by measuring cell survival and skin genotoxicity by measuring MN induction in cultured keratinocytes.

• OSTI ID: 6097864
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 17:3; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
CARBAMATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CELL CONSTITUENTS
CELL NUCLEI
CHRYSENE
CONDENSED AROMATICS
DIMETHYLBENZANTHRACENE
EPIDERMIS
EPITHELIUM
GENETIC EFFECTS
HYDROCARBONS
KERATIN
MAMMALS
METABOLIC ACTIVATION
MICE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRENE
RODENTS
SCLEROPROTEINS
SKIN
TISSUES
URETHANE
VERTEBRATES