Induction of mast cell proliferation, maturation, and heparin synthesis by the rat c-kit ligand, stem cell factor

Journal Article · Mon Jul 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.14.6382· OSTI ID:6096650

Tsai, M; Takeishi, Takashi; Geissler, E N ^[1]; Thompson, H; Metcalfe, D D ^[2]; Langley, K E; Zsebo, K M; Galli, S J ^[3]

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1. Beth Israel Hospital, Boston, MA (United States)
2. National Inst. of Health, Bethesda, MD (United States)
3. Amgen, Inc., Thousand Oaks, CA (United States)

The authors investigated the effects of a newly recognized multifunctional growth factor, the c-kit ligand stem cell factor (SCF), on mouse mast cell proliferation and phenotype. Recombinant rat SCF{sup 164} (rrSCF{sup 164}) induced the development of large numbers of dermal mast cells in normal mice in vivo. Many of these mast cells had features of connective tissue-type mast cells (CTMC), in that they were reactive both with the heparin-binding fluorescent dye berberine sulfate and with safranin. In vitro, rrSCF{sup 164} induced the proliferation of cloned interleukin 3 (IL-3)-dependent mouse mast cells and primary populations of IL-3-dependent, bone marrow-derived cultured mast cells (BMCMC), which represent immature mast cells, and purified peritoneal mast cells, which represent a type of mature CTMC> BMCMC maintained in rrSCF{sup 164} not only proliferated but also matured. These findings identify SCF as a single cytokine that can induce immature, IL-3-dependent mast cells to mature and to acquire multiple characteristics of CTMC. These findings also directly demonstrate that SCF can regulate the development of a cellular lineage expressing c-kit through effects on both proliferation and maturation.

• OSTI ID: 6096650
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:14; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOSYNTHESIS
CARBOHYDRATES
CELL PROLIFERATION
CHEMISTRY
CONNECTIVE TISSUE CELLS
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
HEMATOLOGIC AGENTS
HEPARIN
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
ISOTOPES
LABELLED COMPOUNDS
LIGANDS
LIGHT NUCLEI
MAMMALS
MAST CELLS
MICE
MUCOPOLYSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
RADIOISOTOPES
RODENTS
SACCHARIDES
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
UPTAKE
VERTEBRATES