Carcinogenesis, metabolism and DNA-binding studies of complex organic mixtures

Conference · Mon Oct 01 04:00:00 EDT 1984

OSTI ID:6093575

Springer, D L; Mahlum, D D; Westerberg, R B; Hopkins, K L; Frazier, M E; Later, D W; Weimer, W C

Studies were conducted to determine the skin-tumor-initiating activity of complex mixtures of synfuel derivation. Data from previous studies indicated that solvent refined coal (SRC)-II heavy distillate (HD; boiling range, 550 to > 850/sup 0/F) produced skin tumors in mice and that most of the carcinogenic activity occurred in materials boiling above 750/sup 0/F. Two of the highest-boiling fractions (i.e., 800 to 850/sup 0/F and >850/sup 0/F) contained approximately equal amounts of benzo(a)-pyrene (BaP; 1600 ppM), yet the >850/sup 0/F fraction produced 3.0 times as many skin tumors as the 800 to 850/sup 0/F fraction. Because of these findings, another study was conducted to determine the effects of a complex coal liquid (CL) matrix on the expression of BaP. For this study, BaP was mixed with a carcinogenic CL (50 ..mu..g BaP + 50 mg CL) and applied once to the shaved backs of mice to initiate tumor development. The number of tumors on animals receiving BaP + CL was substantially lower than for animals receiving the same amount of BaP only. These data demonstrate that the initiating activity of BaP was suppressed when it was presented to the animals in a complex chemical matrix. To extend these studies, in vitro and in vivo experiments were conducted to determine the extent of metabolism and DNA-binding of BaP alone or BaP in a complex mixture. 12 references, 5 tables.

• Research Organization: Pacific Northwest Labs., Richland, WA (USA)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6093575
• Report Number(s): PNL-SA-12700; CONF-8410225-3; ON: DE85007153
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BENZOPYRENE
BODY
CARCINOGENESIS
COAL LIQUIDS
CONDENSED AROMATICS
DATA
DISPERSIONS
EXPERIMENTAL DATA
FLUIDS
HYDROCARBONS
INFORMATION
LIQUIDS
MAMMALS
METABOLISM
MICE
MIXTURES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PROMOTERS
RODENTS
SKIN
SYNERGISM
TUMOR PROMOTERS
VERTEBRATES