Tumor necrosis factor induced stimulation of granulopoiesis and radioprotection

Journal Article · · Lymphokine Res.; (United States)

OSTI ID:6087595

Urbaschek, R; Maennel, D N.U.; Urbaschek, B

Human recombinant tumor necrosis factor, TNF, was used to assess its ability to stimulate granulopoiesis and to protect mice against lethal irradiation, effects known to be inducable with TNF-rich postendotoxin serum from BCG infected mice (BCG/ET serum). Although the endotoxin contamination of this TNF preparation is extremely low its effects were compared in endotoxin low responder C3H/HeJ mice and susceptible NMRI mice. TNF is a potent inducer of serum colony stimulating activity, CSA, in both mouse strains. In peripheral blood a marked granulocytosis with a concomitant decrease in lymphocytes and monocytopenia occurs at 2 hours after injection of TNF. Moreover, TNF induces an increase in the number of splenic myelopoietic committed stem cells (GM-CFC, granulocyte-macrophage colony forming cells) determined five days after injection. The lethality rate, registered over 30 days after exposure to 660 cGy whole body X-irradiation is reduced to 40% in C3H/HeJ mice as compared to 75% in control animals. The reduction in lethality is observed both, when TNF was injected 24 hours before or after irradiation. In vitro, TNF significantly increases the number of colonies in the presence of CSA in bone marrow cultures. TNF per se does not effect colony growth. The studies reported here demonstrate that TNF is a myelopoiesis stimulating factor in mice which may be related to the reduction in lethality following whole body irradiation.

• Research Organization: Univ. of Heidelberg, Germany, F.R.
• OSTI ID: 6087595
• Journal Information: Lymphokine Res.; (United States), Journal Name: Lymphokine Res.; (United States) Vol. 6:3; ISSN LYRED
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
IONIZING RADIATIONS
IRRADIATION
LETHAL IRRADIATION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MICE
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIATION PROTECTION
RADIATIONS
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
VERTEBRATES
WHOLE-BODY IRRADIATION
X RADIATION