Substrate diversity of Pseudomonas spp. containing chlorotoluene degradative plasmids
Conference
·
· Dev. Ind. Microbiol.; (United States)
OSTI ID:6082534
Strains of Pseudomonas cepacia and Pseudomonas spp. isolated from soil by direct enrichment with chlorinated toluenes contained plasmids of approx.72 mdaltons which encoded for chlorotoluene degradation. Restriction analyses indicated that these plasmids, though of similar size, are not identical. Unlike Ps. putida mt-2 (TOL), strains used in this investigation did not utilize the meta-cleavage pathway for toluene catabolism. In addition, both the Ps. cepacia and and Pseudomonas spp. exhibit an increased range of activity on mono- and dichloronated toluenes and benzoates. The Ps. cepacia and Pseudomonas spp. were compared also with known toluene-degrading pseudomonads at physiological, biochemical, and molecular levels to gain insight into the development of catabolic plasmids and to explore the potential of such microorganisms to degrade toxic organic compounds. 30 references, 2 figures, 3 tables.
- Research Organization:
- Battelle-Columbus Labs., OH
- OSTI ID:
- 6082534
- Report Number(s):
- CONF-8208164-
- Conference Information:
- Journal Name: Dev. Ind. Microbiol.; (United States) Journal Volume: 24
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550500 -- Metabolism
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
DATA
DECOMPOSITION
EXPERIMENTAL DATA
GENETIC ENGINEERING
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
INFORMATION
METABOLISM
MICROORGANISMS
NUMERICAL DATA
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PLASMIDS
PSEUDOMONAS
TOLUENE
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
DATA
DECOMPOSITION
EXPERIMENTAL DATA
GENETIC ENGINEERING
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
INFORMATION
METABOLISM
MICROORGANISMS
NUMERICAL DATA
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PLASMIDS
PSEUDOMONAS
TOLUENE