Expression and transfer of engineered catabolic pathways harbored by Pseudomonas spp. introuduced into activated sludge microcosms

Journal Article · Thu Oct 01 04:00:00 EDT 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:7006995

Nublein, K; Maris, D; Timmis, K; Dwyer, D F ^[1]

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1. Molecular Microbial Ecology Group, Braunschweig (Germany)

Two genetically engineered microorganisms (GEMs), Pseudomonas sp. strain B13 FR1(pFRC20P) (FR120) and Pseudomonas putida KT2440(pWWO-EB62) (EB62), were introduced into activated sludge microcosms that had the level of aeration, nutrient makeup, and microbial community structure of activated sludge reactors. FR120 contains an experimentally assembled ortho cleavage route for simultaneous degradation of 3-chlorobenzoate (3CB) and 4-methyl benzoate (4MB); EB62 contains a derivative TOL plasmid-encoded degradative pathway for toluene experimentally evolved so that it additionally processes 4-ethyl benzoate (4EB). Experiments assessed survival of the GEMs, their ability to degrade target substrates, and lateral transfer of plasmid-encoded recombinant DNA.

• OSTI ID: 7006995
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:10; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540220 -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
550400* -- Genetics
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
BIOTECHNOLOGY
CHEMICAL REACTIONS
DECOMPOSITION
DNA
GENETIC ENGINEERING
MICROCOSMS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PSEUDOMONAS
RECOMBINANT DNA
SLUDGES
VIABILITY