Biotransformation of substituted benzoates to the corresponding cis-diols by an engineered strain of Pseudomonas oleovorans producing the TOL plasmid-specified enzyme toluate-1,2-dioxygenase

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:7017675

Wubbolts, M G; Timmis, K N ^[1]

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1. Univ. of Geneva (Switzerland)

The conversion of substituted benzoates into 1,2-cis-dihydroxycyclohexa-3,5-diene carboxylic acids (cis-diols) was affected by using Escherichia coli and Pseudomonas recombinants carrying the xylXYZ genes originating from the Pseudomonas putida mt-2 TOL plasmid, thus producing toluate-1,2-dioxygenase. Pseudomonas oleovorans GPo12 recombinants readily produced meta-and para-substituted cis-diols, but were limited in their oxidation of ortho-substituted substrates.

• OSTI ID: 7017675
• Journal Information: Applied and Environmental Microbiology; (USA), Vol. 56:2; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
AROMATICS
BIODEGRADATION
GLYCOLS
BIOSYNTHESIS
ESCHERICHIA COLI
GENES
GENETIC ENGINEERING
OXYGENASES
PLASMIDS
PSEUDOMONAS
RECOMBINANT DNA
ALCOHOLS
BACTERIA
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
DNA
ENZYMES
HYDROXY COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
SYNTHESIS
540220* - Environment
Terrestrial- Chemicals Monitoring & Transport- (1990-)