Biotransformation of substituted benzoates to the corresponding cis-diols by an engineered strain of Pseudomonas oleovorans producing the TOL plasmid-specified enzyme toluate-1,2-dioxygenase
Journal Article
·
· Applied and Environmental Microbiology; (USA)
OSTI ID:7017675
- Univ. of Geneva (Switzerland)
The conversion of substituted benzoates into 1,2-cis-dihydroxycyclohexa-3,5-diene carboxylic acids (cis-diols) was affected by using Escherichia coli and Pseudomonas recombinants carrying the xylXYZ genes originating from the Pseudomonas putida mt-2 TOL plasmid, thus producing toluate-1,2-dioxygenase. Pseudomonas oleovorans GPo12 recombinants readily produced meta-and para-substituted cis-diols, but were limited in their oxidation of ortho-substituted substrates.
- OSTI ID:
- 7017675
- Journal Information:
- Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:2; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
Similar Records
Use of cloned genes of Pseudomonas TOL plasmid to effect biotransformation of benzoates to cis-dihydrodiols and catechols by Escherichia coli cells
Biodegradation of nitrobenzene through a hybrid pathway in Pseudomonas putida
Combination of the tod and the tol pathways in redesigning a metabolic route of Pseudomonas putida for the mineralization of a benzene, toluene, and p-xylene mixture
Journal Article
·
Sat Nov 30 23:00:00 EST 1985
· Appl. Environ. Microbiol.; (United States)
·
OSTI ID:6301010
Biodegradation of nitrobenzene through a hybrid pathway in Pseudomonas putida
Journal Article
·
Tue Dec 19 23:00:00 EST 1995
· Biotechnology and Bioengineering
·
OSTI ID:162987
Combination of the tod and the tol pathways in redesigning a metabolic route of Pseudomonas putida for the mineralization of a benzene, toluene, and p-xylene mixture
Journal Article
·
Thu Jun 01 00:00:00 EDT 1995
· Applied and Environmental Microbiology
·
OSTI ID:96610
Related Subjects
54 ENVIRONMENTAL SCIENCES
540220* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
ALCOHOLS
AROMATICS
BACTERIA
BIODEGRADATION
BIOSYNTHESIS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
DNA
ENZYMES
ESCHERICHIA COLI
GENES
GENETIC ENGINEERING
GLYCOLS
HYDROXY COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PLASMIDS
PSEUDOMONAS
RECOMBINANT DNA
SYNTHESIS
540220* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
ALCOHOLS
AROMATICS
BACTERIA
BIODEGRADATION
BIOSYNTHESIS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
DNA
ENZYMES
ESCHERICHIA COLI
GENES
GENETIC ENGINEERING
GLYCOLS
HYDROXY COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PLASMIDS
PSEUDOMONAS
RECOMBINANT DNA
SYNTHESIS