Biodegradation of ddt (1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane) by the white rot fungus phanerochaete chrysosporium
Extensive biodegradation of 1,1,1-trichloro-2,2bis(4-chlorophenyl)ethane (DDT) by the white rot fungus Phanerochaete chrysosporium was demonstrated by disappearance and mineralization of (14C) DDT in nutrient nitrogen-deficient cultures. Mass balance studies demonstrated the formation of polar and water-soluble metabolites during degradation. Hexane-extractable metabolites identified by gas chromatography-mass spectrometry included 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane(DDD), 2,2,2-trichloro-1,1-bis(4-chlorophenyl)ethanol (dicofol), 2,2-dichloro-1,1-bis(4-chlorophenyl) ethanol (FW-152), and 4,4'-dichlorobenzophenone (DBP). DDD was the first metabolite observed; it appeared after 3 days of incubation and disappeared from culture upon continued incubation. This, as well as the fact that ((14)C) dicofol was mineralized, demonstrates that intermediates formed during DDT degradation are also metabolized. These results demonstrate that the pathway for DDT degradation in P. chrysosporium is clearly different from the major pathway proposed for microbial or environmental degradation of DDT. Like P. chrysosporium ME-446 and BKM-F-1767, the white rot fungi Pleurotus ostreatus, Phellinus weirii, and Polyporus versicolor also mineralized DDT.
- Research Organization:
- Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry
- OSTI ID:
- 6081213
- Report Number(s):
- PB-93-191617/XAB
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALKANES
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIODEGRADATION
BUTYL PHOSPHATES
CARBON 14
CARBON ISOTOPES
CHEMICAL REACTIONS
CHROMATOGRAPHY
CULTURE MEDIA
DBP
DDT
DECOMPOSITION
DEVELOPED COUNTRIES
ELEMENTS
ESTERS
ETHANE
ETHANOL
EUMYCOTA
EVEN-EVEN NUCLEI
FUNGI
GAS CHROMATOGRAPHY
HEXANE
HIGH PRESSURE
HYDROCARBONS
HYDROXY COMPOUNDS
INCUBATION
INSECTICIDES
ISOTOPES
LIGHT NUCLEI
MASS BALANCE
MEASURING INSTRUMENTS
METABOLISM
METABOLITES
MINERALIZATION
NITROGEN
NONMETALS
NORTH AMERICA
NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PESTICIDES
PHANEROCHAETE
PHOSPHORIC ACID ESTERS
PLANTS
POLYPORUS VERSICOLOR
RADIOISOTOPES
SEPARATION PROCESSES
SPECTROMETERS
USA
YEARS LIVING RADIOISOTOPES