Recovery by ultrafiltration of a commercial enzyme for cellulose hydrolysis

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Separation Science and Technology; (United States)

DOI:https://doi.org/10.1080/01496399108050465· OSTI ID:6079413

Pizzichini, M; Fabiani, C; Sperandei, M ^[1]

---

1. ENEA, Rome (Italy)

An enzymatic process of cellulose hydrolysis based mainly on the use of membrane techniques is under study. The proposed flow sheet assumes that during cellulose hydrolysis the enzyme is continuously separated from glucose and cellobiose and is recycled in the cellulose reaction vessel by membrane ultrafiltration. The ultrafiltration of Celluclast enzyme by Novo is performed in a DDS column module assembled with flat polysulfone membranes. Membrane polarization effects are studies in the 0.1-5% w/v enzyme concentration range under varying pressures up to 600 kPa. A partial loss of enzymatic activity is observed as a consequence of the ultrafiltration and membrane washing operations.

• OSTI ID: 6079413
• Journal Information: Separation Science and Technology; (United States), Journal Name: Separation Science and Technology; (United States) Vol. 26:2; ISSN 0149-6395; ISSN SSTED
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090900* -- Biomass Fuels-- Processing-- (1990-)
ALDEHYDES
CARBOHYDRATES
CELLOBIOSE
CELLULOSE
CHEMICAL REACTIONS
DATA
DEACTIVATION
DECOMPOSITION
DIAGRAMS
DISACCHARIDES
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
FILTRATION
FLOWSHEETS
GLUCOSE
HEXOSES
HYDROLYSIS
INFORMATION
LYSIS
MEMBRANES
MONOSACCHARIDES
NUMERICAL DATA
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PERFORMANCE TESTING
POLYSACCHARIDES
PRESSURE DEPENDENCE
PROTEINS
RECYCLING
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
TESTING
ULTRAFILTRATION