Fucosylation of xyloglucan: localization of the transferase in dictyosomes of pea stem cells. [Pisum sativum]

Journal Article · Wed Jul 01 00:00:00 EDT 1987 · Plant Physiol.; (United States)

OSTI ID:6075333

Camirand, A; Brummell, D; MacLachlan, G

Microsomal membranes from elongating regions of etiolated Pisum sativum stems were separated by rate-zonal centrifugation on Renografin gradients. The transfer of labeled fucose and xylose from GDP-(/sup 14/C) fucose and UDP-(/sup 14/C)xylose to xyloglucan occurred mainly in dictyosome-enriched fractions. No transferase activity was detected in secretory vesicle fractions. Pulse-chase experiments using pea stem slices incubated with (/sup 3/H)fucose suggest that xyloglucan chains are fucosylated and their structure completed within the dictyosomes, before being transported to the cell wall by secretory vesicles.

• Research Organization: McGill Univ., Montreal, Quebec
• OSTI ID: 6075333
• Journal Information: Plant Physiol.; (United States), Vol. 84:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
OLIGOSACCHARIDES
BIOSYNTHESIS
SUBCELLULAR DISTRIBUTION
TRANSFERASES
CARBON 14 COMPOUNDS
CELL WALL
CENTRIFUGATION
HEXOSES
MEMBRANES
MICROSOMES
MOLECULAR STRUCTURE
ORGANOIDS
PISUM
PLANT STEMS
TRACER TECHNIQUES
TRANSLOCATION
TRITIUM COMPOUNDS
XYLOSE
ALDEHYDES
BACTERIA
CARBOHYDRATES
CELL CONSTITUENTS
DISTRIBUTION
ENZYMES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGUMINOSAE
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
PLANTS
RHIZOBIUM
SACCHARIDES
SEPARATION PROCESSES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques