Effect of auxin on Golgi-mediated cell wall synthesis in pea stem segments
Stem segments of 7 day-old etiolated Pisum sativum seedlings were abraded using carborundum powder. Batches of segments were pulsed in (/sup 3/H) glucose followed by a chase in cold glucose in the presence or absence of 1AA, then homogenized by chopping with a razor blade. A rate-zonal centrifugation on a linear sucrose gradient was used to separate dictyosomes and secretory vesicles, and membrane-bound radioactivity determined as a measure of Golgi material in the cytoplasm. The amount of membrane-bound radioactivity was increased in tissues treated with 1AA for 30 min, indicative of an enhanced Golgi content in such segments. This increase thus precedes the sustained increase in auxin-stimulated growth of stem segments which occurs around 35-45 min after exposure to auxin and which is thought to be due to increased cell wall synthesis.
- Research Organization:
- McGill Univ., Montreal, Quebec
- OSTI ID:
- 5271686
- Journal Information:
- Plant Physiol., Suppl.; (United States), Journal Name: Plant Physiol., Suppl.; (United States) Vol. 80:4; ISSN PPYSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AUXINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CELL CONSTITUENTS
CELL WALL
GROWTH
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLED POOL TECHNIQUES
LABELLING
LIGHT NUCLEI
NUCLEI
ODD-EVEN NUCLEI
ORGANOIDS
PLANT GROWTH
PLANT GROWTH REGULATORS
PLANT STEMS
RADIOISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM
YEARS LIVING RADIOISOTOPES