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Fucosylation of xyloglucan: localization of the transferase in dictyosomes of pea stem cells. [Pisum sativum]

Journal Article · · Plant Physiol.; (United States)
OSTI ID:6075333

Microsomal membranes from elongating regions of etiolated Pisum sativum stems were separated by rate-zonal centrifugation on Renografin gradients. The transfer of labeled fucose and xylose from GDP-(/sup 14/C) fucose and UDP-(/sup 14/C)xylose to xyloglucan occurred mainly in dictyosome-enriched fractions. No transferase activity was detected in secretory vesicle fractions. Pulse-chase experiments using pea stem slices incubated with (/sup 3/H)fucose suggest that xyloglucan chains are fucosylated and their structure completed within the dictyosomes, before being transported to the cell wall by secretory vesicles.

Research Organization:
McGill Univ., Montreal, Quebec
OSTI ID:
6075333
Journal Information:
Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 84:3; ISSN PLPHA
Country of Publication:
United States
Language:
English