Enzymes from auxin-pretreated pea tissue which hydrolyze xyloglucan and a xyloglucan fragment oligosaccharide
Buffer extracts were prepared from stem tissue of young etiolated pea plants harvested several days after spraying with 2,4-D. Similar extracts were prepared from tissue not pretreated with 2,4-D. Extracts were partially purified by gel filtration to remove carbohydrates. Xyloglucan polymer and a xyloglucan nonasaccharide of known structure were used as substrates. Both substrates contain glucose, xylose, galactose and fucose. The ability of the pea-stem-extracts to enzymatically hydrolyze the glycosidic linkages of these substrates was determined by chemical derivatization of the enzyme reaction products and analysis by gas chromatography-mass spectrometry. Such experiments established that the extracts contained enzymes that hydrolyzed a portion of the glycosidic linkages of each of the four sugar types present in the substrates. Extracts of auxin-pretreated tissue hydrolyzed a greater proportion of the linkages of each type of sugar in the substrates. Work is currently underway to purify and characterize the enzymes responsible for the hydrolytic activities observed.
- Research Organization:
- Univ. of Georgia, Athens
- OSTI ID:
- 5493754
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 80:4; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AUXINS
BACTERIA
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
ENZYMATIC HYDROLYSIS
GAS CHROMATOGRAPHY
HYDROLYSIS
LEGUMINOSAE
LYSIS
MASS SPECTROSCOPY
MICROORGANISMS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PISUM
PLANT GROWTH REGULATORS
PLANT TISSUES
PLANTS
POLYSACCHARIDES
RHIZOBIUM
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
SPECTROSCOPY
TISSUES