Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Different mutations are responsible for the elevated sister chromatid exchange frequencies characteristic of Bloom's syndrome and hamster EM9 cells

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ;
Experimental hybridization of cultured cells was employed to determine whether the strikingly elevated rates of sister-chromatid exchange (SCE) exhibited by Bloom's syndrome (BS) and hamster cell line EM9 have the same or different bases. Seventeen cell lines were developed from polyethylene glycol-treated mixtures of BS and EM9 cells. Cytogenetic analysis proved the hybrid nature of 12 of the lines; 9 of those 12 exhibited low (normal) numbers of SCEs, signifying complementation. The parental BS and EM9 cells, although resembling each other in exhibiting very high SCE frequencies in BrdUrd-containing medium, differ from one another with respect to their proliferative abilities in such medium, the EM9 cells but not the BS cells being exquisitely hypersensitive to BrdUrd. In the low-SCE hybrid lines, hypersensitivity to growth in BrdUrd-containing medium was restored to normal whereas the hypersensitivity was retained by the high-SCE hybrids. It is concluded, first, that the mutations in BS and EM9 cells are different and, second, that both the elevated SCE frequency and the excessive BrdUrd hypersensitivity of EM9 cells are due to the same mutation.
Research Organization:
New York Blood Center, NY
OSTI ID:
6072106
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Journal Issue: 8 Vol. 84:8; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

DNA-mediated transfer of a human DNA repair gene that controls sister chromatid exchange
Journal Article · Sun Mar 31 23:00:00 EST 1985 · Mol. Cell. Biol.; (United States) · OSTI ID:6194070
Somatic intragenic recombination within the mutated locus BLM can correct the high sister-chromatid exchange phenotype of Bloom syndrome cells
Journal Article · Tue Oct 31 23:00:00 EST 1995 · American Journal of Human Genetics · OSTI ID:209892
In vivo persistence of sister chromatid exchanges (SCE) induced by gamma rays in mouse bone marrow cells
Journal Article · Sat Dec 31 23:00:00 EST 1983 · Environ. Mutagen.; (United States) · OSTI ID:6221340

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIMETABOLITES
AZINES
BIOLOGY
BROMOURACILS
BUDR
CELL CULTURES
CELL PROLIFERATION
CHROMOSOMAL ABERRATIONS
CYTOLOGY
DISEASES
DRUGS
HAMSTERS
HEREDITARY DISEASES
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
HYDROXY COMPOUNDS
MAMMALS
MAN
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOGENESIS
PRIMATES
PYRIMIDINES
RIBOSIDES
RODENTS
SENSITIVITY
SISTER CHROMATID EXCHANGES
URACILS
VERTEBRATES