DNA-mediated transfer of a human DNA repair gene that controls sister chromatid exchange
Journal Article
·
· Mol. Cell. Biol.; (United States)
The Chinese hamster cell line mutants EM9, which has a reduced ability to repair DNA strand breaks, is noted for its highly elevated frequency of sister chromatid exchange, a property shared with cells from individuals with Bloom's syndrome. The defect in EM9 cells was corrected by fusion hybridization with normal human fibroblasts and by transfection with DNA from hybrid cells. The transformants showed normalization of sister chromatid exchange frequency but incomplete correction of the repair defect in terms of chromosomal aberrations produced by 5-bromo-2-deoxyuridine. 28 references, 1 figure, 1 table.
- Research Organization:
- Lawrence Livermore Lab., CA
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 6194070
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 5:4; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Different mutations are responsible for the elevated sister chromatid exchange frequencies characteristic of Bloom's syndrome and hamster EM9 cells
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Fri Dec 31 23:00:00 EST 1993
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OSTI ID:5483028
Different mutations are responsible for the elevated sister chromatid exchange frequencies characteristic of Bloom's syndrome and hamster EM9 cells
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· Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Cells from an immunodeficient patient (46BR) with a defect in DNA ligation are hypomutable but hypersensitive to the induction of sister chromatid exchanges
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· Proc. Natl. Acad. Sci. U.S.A.; (United States)
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OSTI ID:6377676
Related Subjects
550400* -- Genetics
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIMETABOLITES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHO CELLS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DATA
DEOXYURIDINE
DNA
DNA REPAIR
DRUGS
EXPERIMENTAL DATA
FIBROBLASTS
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
HYDROXY COMPOUNDS
INFORMATION
MUTANTS
MUTATIONS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RECOVERY
REPAIR
RIBOSIDES
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
STRAND BREAKS
URACILS
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIMETABOLITES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHO CELLS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DATA
DEOXYURIDINE
DNA
DNA REPAIR
DRUGS
EXPERIMENTAL DATA
FIBROBLASTS
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
HYDROXY COMPOUNDS
INFORMATION
MUTANTS
MUTATIONS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RECOVERY
REPAIR
RIBOSIDES
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
STRAND BREAKS
URACILS