In vitro macro- and micro-autoradiographic localization of atrial natriuretic peptide in the rat kidney

Journal Article · Fri May 01 00:00:00 EDT 1987 · Res. Commun. Chem. Pathol. Pharmacol.; (United States)

OSTI ID:6071495

Yamamoto, I; Ogura, T; Ota, Z

We investigated the receptor localization of atrial natriuretic peptide (ANP) in the rat kidney, by in vitro macro- and micro-autoradiography (ARG) of (/sup 125/I)-ANP using nonfixed frozen sections. First, we examined the optimum conditions for ARG with respect to the effects of polyethylenimine (PEI), thickness of section, incubation time and degradation of (/sup 125/I)-ANP. Saturation experiments of (/sup 125/I)-ANP using rat kidney sections revealed the presence of high affinity binding sites of (/sup 125/I)-ANP in the renal cortex (Kd = 0.52 nM). Macro-autoradiograms showed that the dense grains representing specific binding sites of (/sup 125/I)-ANP were distributed in the cortex in a punctate pattern. Using micro-autoradiography, the localization of the dense grains on the emulsion was compared with the staining pattern of the same section subjected to double staining. In the renal cortex, the dense grains were observed on the glomerulus, blood vessels and proximal tubules. Dense grains were also observed in the mesangium area of glomeruli, the inside wall of blood vessels (especially endothelium), and the inside wall of proximal tubules (possibly brush border). These results suggested that the physiological action of ANP in the kidney is mediated by its receptors. Also, ARG was useful for accurately detecting the action sites of ANP.

• Research Organization: Okayama Univ. Medical School, Japan
• OSTI ID: 6071495
• Journal Information: Res. Commun. Chem. Pathol. Pharmacol.; (United States), Vol. 56:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PEPTIDES
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
IN VITRO
IODINE 125
KIDNEYS
RATS
RECEPTORS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques