Free radical formation during in vivo metabolism of Freon 11 (CCl/sub 3/F) and 1,1,1-trichloroethane

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6068666

Poyer, J L; Downs, P; Massion, W

Free radical products were identified by electron paramagnetic resonance (EPR) techniques in rat liver lipid extracts 2 hours after administering the refrigerant Freon 11, CCl/sub 3/F (3-7% in air) or the industrial solvent 1,1,1-trichloroethane (0.5%) by inhalation. Phenyl-t-butyl nitrone (PBN) was used as the spin-trapping agent and was given in a corn oil-phosphate buffer emulsion by stomach tube prior to exposure to CCl/sub 3/F or 1,1,1-trichloroethane. The EPR spectrum of the PBN-trapped radical derived from CCl/sub 3/F metabolism, consisted of a triplet of triplets. This indicates the presence of a fluorine atom attached to the trapped carbon-centered radical. Splitting constants for the PBN-trapped radical derived from 1,1,1-trichloroethane metabolism were A/sub N/=14.81 G and A/sup H/=2.08 G. When metyrapone, an inhibitor of the 52 kd liver cyt. P-450, was administered i.p. one hour before the PBN and exposure to the CCl/sub 3/F or 1,1,1-trichloroethane, the formation of PBN-trapped radicals was inhibited 80%.

• Research Organization: Univ. of Oklahoma Health Sciences Center, Oklahoma City
• OSTI ID: 6068666
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHLORINATED ALIPHATIC HYDROCARBONS
ELECTRON SPIN RESONANCE
FREONS
HALOGENATED ALIPHATIC HYDROCARBONS
IN VIVO
MAGNETIC RESONANCE
METABOLISM
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
RADICALS
RESONANCE