ESR spin trapping study of the effect of oxidative stress and dietary Zn deficiency on free radical production in vivo

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5174078

Xu, Z; Chen, G; Bray, T M ^[1]

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1. Univ. of Guelph, Ontario (Canada)

The objective of this study is to examine if free radicals can be trapped in vivo in dietary Zn deficient rats using ESR spin trapping techniques. The ability of Zn deficient rats to cope with oxidative stress is also assessed. {alpha}-Phenyl-N-t-butyl-nitrone (PBN), a spin trap, was i.p injected at a dose of 75 mg or 150 mg/kg body weight. The tissue distribution and metabolism of PBN in Zn deficient, pair-fed and control rats were compared. ESR signals in liver and lung were used as a measure of free radicals generated in vivo. When the rats were challenged with free radical generating agents, CCl{sub 4}, or FeNTA, the intensity of ESR signal recorded from the Zn deficient rats was about 50% and 30% of its pair-fed and ad libitum control, respectively. Under other oxidative stress, such as exposure to ethanol and hyperoxia, there was a higher production of free radicals in the Zn deficient rats compared to its control. These results suggest that dietary Zn deficiency may either increase the susceptibility or reduce the defense ability of the animal to increased oxidative stress.

• OSTI ID: 5174078
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMALS
ANOXIA
BIOLOGICAL EFFECTS
BIOLOGICAL STRESS
BIOSYNTHESIS
DIET
DISTRIBUTION
ELECTRON SPIN RESONANCE
ELEMENTS
ETHANOL
HYDROXY COMPOUNDS
IN VIVO
MAGNETIC RESONANCE
MAMMALS
METABOLISM
METALS
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
RADICALS
RATS
RESONANCE
RODENTS
SYNTHESIS
TISSUE DISTRIBUTION
VERTEBRATES
ZINC