Displacement chromatography applied to trace component analysis

Journal Article · Sat Dec 01 04:00:00 EST 1990 · Analytical Chemistry (Washington); (USA)

DOI:https://doi.org/10.1021/ac00222a009· OSTI ID:6068403

Ramsey, R; Katti, A M; Guiochon, G ^[1]

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1. Univ. of Tennessee, Knoxville (USA) Oak Ridge National Laboratory, TN (USA)

Displacement chromatography has been used primarily for the isolation of relatively large quantities of materials in preparative scale separations. The authors show that it also offers advantages for the enrichment of trace components. During displacement development, significant compression of the trace component bands occurs. This enrichment is studied both experimentally and theoretically. The theoretical model is based on the solution of the mass balance equations for nonlinear chromatography, assuming competitive Langmuir isotherms. The system studied experimentally consisted of parts-per-million levels of {beta}-naphthylamine and an impurity of the naphthylamine as the sample and diethyl phthalate as the displacer. The band profiles of the trace components were monitored by fluorescence detection while the displacer was monitored by UV absorbance. Wavelengths were chosen such that the profiles of the sample and the displacer could be monitored independently. Trace enrichment by band compression was achieved by increasing the displacer concentration. Experimental results show very narrow bands at enhanced concentrations as compared to the relatively broad Gaussian-shaped profiles observed in linear elution chromatography. The experimental results are in agreement with theoretical predictions of peak shape.

• OSTI ID: 6068403
• Journal Information: Analytical Chemistry (Washington); (USA), Journal Name: Analytical Chemistry (Washington); (USA) Journal Issue: 23 Vol. 62:23; ISSN 0003-2700; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
AMINES
CALCULATION METHODS
CHROMATOGRAPHY
DATA
EXPERIMENTAL DATA
EXTRACTION APPARATUSES
EXTRACTION COLUMNS
INFORMATION
LIQUID COLUMN CHROMATOGRAPHY
MEASURING METHODS
NUMERICAL DATA
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC AMINES
SEPARATION EQUIPMENT
SEPARATION PROCESSES
THEORETICAL DATA
TRACE AMOUNTS