Induced rates of mitotic crossing over and possible mitotic gene conversion per wing anlage cell in Drosophila melanogaster by X rays and fission neutrons

Journal Article · Sat Sep 01 04:00:00 EDT 1990 · Genetics; (USA)

OSTI ID:6067466

Ayaki, T; Fujikawa, K; Ryo, H; Itoh, T; Kondo, S ^[1]

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1. Nagasaki Univ. School of Medicine (Japan)

As a model for chromosome aberrations, radiation-induced mitotic recombination of mwh and flr genes in Drosophila melanogaster strain (mwh +/+ flr) was quantitatively studied. Fission neutrons were five to six times more effective than X rays per unit dose in producing either crossover-mwh/flr twins and mwh singles-or flr singles, indicating that common processes are involved in the production of crossover and flr singles. The X-ray-induced rate/wing anlage cell/Gy for flr singles was 1 X 10(-5), whereas that of crossover was 2 x 10(-4); the former and the latter rate are of the same order of magnitude as those of gene conversion and crossover in yeast, respectively. Thus, we conclude that proximal-marker flr singles induced in the transheterozygote are gene convertants. Using the model based on yeast that recombination events result from repair of double-strand breaks or gaps, we propose that mitotic recombination in the fly is a secondary result of recombinational DNA repair. Evidence for recombinational misrepair in the fly is given. The relative ratio of radiation-induced mitotic crossover to spontaneous meiotic crossover is one order of magnitude higher in the fly than in yeast and humans.

• OSTI ID: 6067466
• Journal Information: Genetics; (USA), Journal Name: Genetics; (USA) Vol. 126:1; ISSN 0016-6731; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ARTHROPODS
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL DIVISION
CHROMOSOMAL ABERRATIONS
DIPTERA
DNA REPAIR
DROSOPHILA
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
EUMYCOTA
FERMIONS
FISSION NEUTRONS
FLIES
FRUIT FLIES
FUNGI
GENE MUTATIONS
GENE RECOMBINATION
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HADRONS
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
MICROORGANISMS
MITOSIS
MUTATIONS
NEUTRONS
NUCLEONS
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RBE
RECOVERY
REPAIR
STRAND BREAKS
X RADIATION
YEASTS