Enzymatic hydrolysis of cellulosic materials: a kinetic study

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260261013· OSTI ID:6066652

Beltrame, P L; Carniti, P; Focher, B; Marzetti, A; Sarto, V

A kinetic study of the enzymatic hydrolysis of two celluloses with different structural features was performed at various temperatures (26-50/sup 0/C). The enzymatic system consisted of three types of enzymes: E/sub 1/-..beta..-1,4-glucan glucanohydrolase; E/sub 2/-..beta..-1,4-glucan cellobiohydrolase; and E/sub 3/-..beta..-glucosidase. A mathematical model for the mechanism of the hydrolysis of cellulosic materials catalyzed by a multienzymatic system was checked and a good rationalization of the experimental results was achieved. Uncompetitive and competitive glucose inhibition on E/sub 1/ and E/sub 2/, respectively, appeared to occur for both substrates. Inhibition by cellobiose was checked at 34/sup 0/C on one substrate. The V/sub max/, K/sub m/, and glucose inhibition constants were optimized and their dependence on temperature determined.

• Research Organization: Universita di Milano, Italy
• OSTI ID: 6066652
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 26:10; ISSN BIBIA
• Country of Publication: United States
• Language: English

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ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
CELLOBIOSE
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ENZYMATIC HYDROLYSIS
ENZYMES
GLUCOSE
HEXOSES
HYDROLYSIS
INHIBITION
LYSIS
MATHEMATICAL MODELS
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
POLYSACCHARIDES
RESEARCH PROGRAMS
SACCHARIDES
SOLVOLYSIS
SYNERGISM
TEMPERATURE DEPENDENCE