Study of the enzymatic hydrolysis of cellulose for production of fuel ethanol by the simultaneous saccharification and fermentation process
- National Renewable Energy Lab., Golden, CO (United States)
The biochemical conversion of cellulosic biomass to ethanol, a promising alternative fuel, can be carried out efficiently and economically using the simultaneous saccharification and fermentation (SSF) process. The SSF integrates the enzymatic hydrolysis of cellulose to glucose, catalyzed by the synergistic action of cellulase and [beta]-glucosidase, with the fermentative synthesis of ethanol. Because the enzymatic step determines the availability of glucose to the ethanologenic fermentation, the kinetics of cellulose hydrolysis by cellulase and [beta]-glucosidase and the susceptibility of the two enzymes to inhibition by hydrolysis and fermentation products are of significant importance to the SSF performance and were investigated under realistic SSF conditions. A previously developed SSF mathematical model was used to conceptualize the depolymerization of cellulose. The model was regressed to the collected data to determine the values of the enzyme parameters and was found to satisfactorily predict the kinetics of cellulose hydrolysis. Cellobiose and glucose were identified as the strongest inhibitors of cellulase and [beta]-glucosidase, respectively. Experimental and modeling results are presented in light of the impact of enzymatic hydrolysis on fuel ethanol production.
- OSTI ID:
- 6806759
- Journal Information:
- Biotechnology and Bioengineering; (United States), Vol. 41:9; ISSN 0006-3592
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELLULOSE
ENZYMATIC HYDROLYSIS
ETHANOL
YIELDS
CELLULASE
FERMENTATION
GLUCOSIDASE
MATHEMATICAL MODELS
SACCHARIFICATION
ALCOHOLS
BIOCONVERSION
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
HYDROXY COMPOUNDS
LYSIS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
SOLVOLYSIS
090900* - Biomass Fuels- Processing- (1990-)