Simultaneous saccharification and fermentation of cellulose to ethanol

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1981

OSTI ID:6273861

Shea, P T

Simultaneous saccharification and fermentation (SSF) of cullulose (untreated BW-200 Solka Floc) to ethanol utilizing the cellulase enzyme complex of Trichoderma reesei Rut C-30 and the yeast Saccharomyces cerevisiae QM 8226, has resulted in increased rates and longer times of hydrolysis when compared to simple saccharifications. Additionally, two schemes for ethanol removal during hydrolysis, nitrogen sparging and vacuum operation, have also shown increased rates and longer times of saccharification of cellulose when compared to the simple SSF. Both early and delayed yeast additions, different lengths of SSF operations, and different sparging techniques were investigated. The beta-glucosidase fraction of the T. ressei Rut C-30 cellulase enzyme system was able to convert cellobiose to glucose in the presence of ethyl alcohol eliminating the strong inhibition of celloboise on cellulase while the yeast converted glucose to ethanol by glucolysis eliminating the inhibition of glucose on beta-glucosidase. The hydrolysis curves did not fit either simple or competitive product inhibition Michaelis-Menten type kinetic analysis. An enzyme deactivation-inhibition model seems necessary to fit the data. The yield parameter for ethanol/substrate (Yp/s) varied from .42g/g to .47g/g (theoretical .51g/g) with the majority of glucose being converted to ethanol in less than 15 hours.

• OSTI ID: 6273861
• Country of Publication: United States
• Language: English

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ALCOHOLS
ALDEHYDES
BIOCONVERSION
BIOSYNTHESIS
CARBOHYDRATES
CELLULOSE
CHEMICAL REACTIONS
CONTINUOUS CULTURE
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ETHANOL
FERMENTATION
FUNGI
GLUCOSE
HEXOSES
HYDROLYSIS
HYDROXY COMPOUNDS
LYSIS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SACCHARIFICATION
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SOLVOLYSIS
SYNTHESIS
TRICHODERMA
TRICHODERMA VIRIDE
YEASTS